Wei-Ping Chen1, Zhong-Nan Hu2, Li-Bin Jin1, Li-Dong Wu1. 1. Department of Orthopedics Surgery, The Second Hospital of Medical College, Zhejiang University, Hangzhou, China. 2. Department of Orthopedics Surgery, The Children's Hospital of Medical College, Zhejiang University, Hangzhou, China.
Abstract
BACKGROUND: Osteoarthritis (OA) is a joint disease in which cartilage degradation is the central pathological change. In this study, we investigated the anti-osteoarthritic effects of licochalcone A (Lico A) in rat chondrocytes. METHODS: Polymerase chain reaction and Western blotting were performed to evaluate the expression of a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)-5, ADAMTS-4, collagen II, matrix metalloproteinase (MMP)-13 and MMP-1 at both the gene and protein levels, respectively. In addition, the wnt/β-catenin and nuclear factor kappa B (NF-κB) signaling pathways were also analyzed by Western blotting. RESULTS: Lico A downregulated ADAMTS-5, ADAMTS-4,MMP-13 and MMP-1 expression, and diminished the IL-1β-induced inhibition of collagen II. In addition, the activation of β-catenin and phosphorylation of p65 and IKKα/β were suppressed by Lico A. CONCLUSIONS: Our results suggest that Lico A inhibits MMPs and ADAMTS partly via the NF-κB and wnt/β-catenin signaling pathways in rat chondrocytes. Thus, Lico A may have therapeutic effects in OA.
BACKGROUND:Osteoarthritis (OA) is a joint disease in which cartilage degradation is the central pathological change. In this study, we investigated the anti-osteoarthritic effects of licochalcone A (Lico A) in rat chondrocytes. METHODS: Polymerase chain reaction and Western blotting were performed to evaluate the expression of a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)-5, ADAMTS-4, collagen II, matrix metalloproteinase (MMP)-13 and MMP-1 at both the gene and protein levels, respectively. In addition, the wnt/β-catenin and nuclear factor kappa B (NF-κB) signaling pathways were also analyzed by Western blotting. RESULTS:Lico A downregulated ADAMTS-5, ADAMTS-4,MMP-13 and MMP-1 expression, and diminished the IL-1β-induced inhibition of collagen II. In addition, the activation of β-catenin and phosphorylation of p65 and IKKα/β were suppressed by Lico A. CONCLUSIONS: Our results suggest that Lico A inhibits MMPs and ADAMTS partly via the NF-κB and wnt/β-catenin signaling pathways in rat chondrocytes. Thus, Lico A may have therapeutic effects in OA.
Authors: Shi Sirong; Chen Yang; Tian Taoran; Li Songhang; Lin Shiyu; Zhang Yuxin; Shao Xiaoru; Zhang Tao; Lin Yunfeng; Cai Xiaoxiao Journal: Bone Res Date: 2020-02-10 Impact factor: 13.567
Authors: Shi Sirong; Chen Yang; Tian Taoran; Li Songhang; Lin Shiyu; Zhang Yuxin; Shao Xiaoru; Zhang Tao; Lin Yunfeng; Cai Xiaoxiao Journal: Bone Res Date: 2020-02-10 Impact factor: 13.567
Authors: Elizabeth Barrionuevo; Florencia Cayrol; Graciela A Cremaschi; Patricia G Cornier; Dora B Boggián; Carina M L Delpiccolo; Ernesto G Mata; Leonor P Roguin; Viviana C Blank Journal: Front Pharmacol Date: 2020-02-25 Impact factor: 5.810