Literature DB >> 28957496

Probability Map Viewer: near real-time probability map generator of serial block electron microscopy collections.

Christopher Churas1,2, Alex J Perez1,2, Hiroyuki Hakozaki1, Willy Wong1, David Lee1, Steven T Peltier1,2, Mark H Ellisman1,2,3.   

Abstract

SUMMARY: To expedite the review of semi-automated probability maps of organelles and other features from 3D electron microscopy data we have developed Probability Map Viewer, a Java-based web application that enables the computation and visualization of probability map generation results in near real-time as the data are being collected from the microscope. Probability Map Viewer allows the user to select one or more voxel classifiers, apply them on a sub-region of an active collection, and visualize the results as overlays on the raw data via any web browser using a personal computer or mobile device. Thus, Probability Map Viewer accelerates and informs the image analysis workflow by providing a tool for experimenting with and optimizing dataset-specific segmentation strategies during imaging.
AVAILABILITY AND IMPLEMENTATION: https://github.com/crbs/probabilitymapviewer. CONTACT: mellisman@ucsd.edu. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.
© The Author 2017. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com

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Year:  2017        PMID: 28957496      PMCID: PMC5870650          DOI: 10.1093/bioinformatics/btx376

Source DB:  PubMed          Journal:  Bioinformatics        ISSN: 1367-4803            Impact factor:   6.937


  9 in total

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  9 in total

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