Yanjun Chen1, Xinjun Wang. 1. Department of General Surgery, the Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, 450052 China.
Abstract
PURPOSE: To study the astrocyte elevated gene (AEG)-1 in breast cancer and the mechanism of the chemokine receptor CXCR4 regulating the epithelial-mesenchymal transition (EMT) involved in brain metastases of breast cancer. METHODS: A total of 20 breast cancer patients with and 40 without brain metastases were recruited. The expressions of AEG-1, CXCR4, E-cadherin, N-cadherin and α-SMA were detected by immunohistochemical staining, real-time (RT) quantitative (q) PCR and Western blot respectively in cancer and adjacent normal tissues. RESULTS: The expressions detected in the adjacent normal tissues from both groups showed no significant difference (p>0.05). In the group with brain metastases, AEG-1, CXCR4, N-cadherin, α-SMA, mRNA and the relative expression level of protein were higher than those of patients without brain metastases, while E-cadherin showed the opposite trend. CONCLUSION: AEG-1 and CXCR4 activate and regulate the EMT pathway to participate in brain metastases.
PURPOSE: To study the astrocyte elevated gene (AEG)-1 in breast cancer and the mechanism of the chemokine receptor CXCR4 regulating the epithelial-mesenchymal transition (EMT) involved in brain metastases of breast cancer. METHODS: A total of 20 breast cancerpatients with and 40 without brain metastases were recruited. The expressions of AEG-1, CXCR4, E-cadherin, N-cadherin and α-SMA were detected by immunohistochemical staining, real-time (RT) quantitative (q) PCR and Western blot respectively in cancer and adjacent normal tissues. RESULTS: The expressions detected in the adjacent normal tissues from both groups showed no significant difference (p>0.05). In the group with brain metastases, AEG-1, CXCR4, N-cadherin, α-SMA, mRNA and the relative expression level of protein were higher than those of patients without brain metastases, while E-cadherin showed the opposite trend. CONCLUSION:AEG-1 and CXCR4 activate and regulate the EMT pathway to participate in brain metastases.
Authors: Ilya V Ulasov; Anton V Borovjagin; Peter Timashev; Massimo Cristofanili; Danny R Welch Journal: Cancer Metastasis Rev Date: 2019-09 Impact factor: 9.264