Yeon-Jee Yoo1, Ikyung Kwon1, So-Ram Oh2, Hiran Perinpanayagam3, Sang-Min Lim1, Ki-Bum Ahn4, Yoon Lee5, Seung-Hyun Han4, Seok-Woo Chang2, Seung-Ho Baek1, Qiang Zhu6, Kee-Yeon Kum7. 1. Department of Conservative Dentistry, Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Republic of Korea. 2. Department of Conservative Dentistry, School of Dentistry, Kyung Hee University, Seoul, Republic of Korea. 3. Division of Restorative Dentistry, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Canada. 4. Department of Oral Microbiology and Immunology, Dental Research Institute and BK21 Plus Program, School of Dentistry, Seoul National University, Seoul, Republic of Korea. 5. Department of Conservative Dentistry, Wonju Severance Christian Hospital, Yonsei University, Wonju, Republic of Korea. 6. Division of Endodontology, Department of Oral Health and Diagnostic Sciences, School of Dental Medicine, University of Connecticut Health Center, Farmington, Connecticut. 7. Department of Conservative Dentistry, Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Republic of Korea. Electronic address: kum6139@snu.ac.kr.
Abstract
INTRODUCTION: The aim of this study was to assess the antifungal efficacy of a synthetic human beta-defensin-3-C15 peptide (HBD3-C15) in Candida albicans-infected human root dentin. METHODS: Standardized root dentin blocks were prepared (6-mm thick, 0.7-mm-wide canal) from single-rooted human permanent premolars and infected with C. albicans for 3 weeks. They were randomly divided into 4 groups (n = 8/group), and their canals were filled with calcium hydroxide (CH), HBD3-C15 peptide, or chlorhexidine digluconate (CHX, 2%) as disinfectants or saline as control. After 1 week of disinfection, dentinal debris were harvested at depths of 200 and 400 μm from the canal lumen, and incubated in Yeast broth for 72 hours at 37°C. Then, colony-forming units (CFU) were measured to assess the antifungal efficacy of each medicament and analyzed statistically. RESULTS: All medicaments showed significantly lower CFU than saline (P < .05), and their antifungal efficacies were similar at both 200- and 400-μm tubular depths (P > .05). HBD3-C15 had similar antifungal efficacy to that of CHX at both depths (P > .05), and both medicaments had significantly lower CFU than CH at both depths (P < .05). CONCLUSIONS: In this ex vivo model of C. albicans-infected human root dentin, the antifungal efficacy of synthetic HBD3-C15 was comparable with CHX.
RCT Entities:
INTRODUCTION: The aim of this study was to assess the antifungal efficacy of a synthetic humanbeta-defensin-3-C15 peptide (HBD3-C15) in Candida albicans-infected human root dentin. METHODS: Standardized root dentin blocks were prepared (6-mm thick, 0.7-mm-wide canal) from single-rooted human permanent premolars and infected with C. albicans for 3 weeks. They were randomly divided into 4 groups (n = 8/group), and their canals were filled with calcium hydroxide (CH), HBD3-C15 peptide, or chlorhexidine digluconate (CHX, 2%) as disinfectants or saline as control. After 1 week of disinfection, dentinal debris were harvested at depths of 200 and 400 μm from the canal lumen, and incubated in Yeast broth for 72 hours at 37°C. Then, colony-forming units (CFU) were measured to assess the antifungal efficacy of each medicament and analyzed statistically. RESULTS: All medicaments showed significantly lower CFU than saline (P < .05), and their antifungal efficacies were similar at both 200- and 400-μm tubular depths (P > .05). HBD3-C15 had similar antifungal efficacy to that of CHX at both depths (P > .05), and both medicaments had significantly lower CFU than CH at both depths (P < .05). CONCLUSIONS: In this ex vivo model of C. albicans-infected human root dentin, the antifungal efficacy of synthetic HBD3-C15 was comparable with CHX.
Authors: Nicholas G Fischer; Eliseu A Münchow; Candan Tamerler; Marco C Bottino; Conrado Aparicio Journal: J Mater Chem B Date: 2020-08-04 Impact factor: 6.331