Jie Gao1, Meng Wang2, Linting Wei1, Dan Niu3, Jiali Wei4, Yan Ou1, Tianbo Jin5, Qiaoling Yu6, Xinghan Liu2, Tian Tian2, Cong Dai2, Rongguo Fu1, Li Wang1. 1. Department of Nephrology, Second Affiliated Hospital of Xi'an, Xi'an, China. 2. Department of Oncology, Second Affiliated Hospital of Xi'an, Xi'an, China. 3. Department of Nephrology, First Affiliated Hospital of Xi'an, Jiaotong University, Xi'an, China. 4. Department of Nephrology, Hainan general hospital, Haikou, China. 5. National Engineering Research Center for Miniaturized Detection Systems, School of Life Sciences, Northwest University, Xi'an, China. 6. Department of Pathology, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.
Abstract
BACKGROUND/AIMS: Endothelial nitric oxide synthase (eNOS) is one of the most important enzymes for producting nitric oxide (NO), which regulate the function of many organs and cells. The single nucleotide polymorphisms (SNPs) of eNOS were found to be associated with many kidney diseases. However, it is lack of relevant studies to evaluate the associations between eNOS polymorphisms and immunoglobulin A nephropathy (IgAN). This case-control study aimed to evaluate the relationship between eNOS polymorphisms and IgAN. METHODS: We recruited 351 IgAN patients and 310 age- and sex-matched healthy controls from Northwest China. Sequenom MassARRAY was used to detect the genotypes of two common eNOS SNPs (rs1799983 and rs2070744). Odds ratios (ORs) and 95% confidence intervals (95% CIs) were calculated by the Chi square test to evaluate the associations between eNOS and IgAN. Phase 2.1 was used to conduct haplotype analysis. RESULTS: In the overall analysis, we found that the rs1799983 polymorphism was associated with a decreased risk of IgAN (G/T vs. G/G: OR=0.57, 95%CI=0.34-0.96; G/T+T/T vs. G/G: OR=0.52, 95%CI=0.31-0.86; G/T vs. G/G-T/T: OR=0.60, 95%CI=0.36-0.99; Log-additive model: OR=0.48, 95%CI=0.30-0.78). Haplotype analysis indicated that Trs1799983Crs2070744 is a protective factor against IgAN (OR=0.62, 95%CI=0.42--0.92). However, no significant differences were found between the two SNPs (rs1799983 and rs2070744) and clinical features (age, sex, blood pressure, and Lee's grade) of IgAN. CONCLUSION: The eNOS gene rs1799983 polymorphism and Trs1799983Crs2070744 haplotype may reduce the risk of IgAN in Chinese populations.
BACKGROUND/AIMS: Endothelial nitric oxide synthase (eNOS) is one of the most important enzymes for producting nitric oxide (NO), which regulate the function of many organs and cells. The single nucleotide polymorphisms (SNPs) of eNOS were found to be associated with many kidney diseases. However, it is lack of relevant studies to evaluate the associations between eNOS polymorphisms and immunoglobulin A nephropathy (IgAN). This case-control study aimed to evaluate the relationship between eNOS polymorphisms and IgAN. METHODS: We recruited 351 IgANpatients and 310 age- and sex-matched healthy controls from Northwest China. Sequenom MassARRAY was used to detect the genotypes of two common eNOS SNPs (rs1799983 and rs2070744). Odds ratios (ORs) and 95% confidence intervals (95% CIs) were calculated by the Chi square test to evaluate the associations between eNOS and IgAN. Phase 2.1 was used to conduct haplotype analysis. RESULTS: In the overall analysis, we found that the rs1799983 polymorphism was associated with a decreased risk of IgAN (G/T vs. G/G: OR=0.57, 95%CI=0.34-0.96; G/T+T/T vs. G/G: OR=0.52, 95%CI=0.31-0.86; G/T vs. G/G-T/T: OR=0.60, 95%CI=0.36-0.99; Log-additive model: OR=0.48, 95%CI=0.30-0.78). Haplotype analysis indicated that Trs1799983Crs2070744 is a protective factor against IgAN (OR=0.62, 95%CI=0.42--0.92). However, no significant differences were found between the two SNPs (rs1799983 and rs2070744) and clinical features (age, sex, blood pressure, and Lee's grade) of IgAN. CONCLUSION: The eNOS gene rs1799983 polymorphism and Trs1799983Crs2070744 haplotype may reduce the risk of IgAN in Chinese populations.
Authors: M Boronat; A Tugores; P Saavedra; P Garay; E Bosch; D Lorenzo; A Ibarra; C García-Cantón Journal: Acta Endocrinol (Buchar) Date: 2021 Oct-Dec Impact factor: 1.104
Authors: Elena García-Martín; Santiago Navarro-Muñoz; Christopher Rodriguez; Mercedes Serrador; Hortensia Alonso-Navarro; Marisol Calleja; Laura Turpín-Fenoll; Marta Recio-Bermejo; Rafael García-Ruiz; Jorge Millán-Pascual; Francisco Navacerrada; José Francisco Plaza-Nieto; Esteban García-Albea; José A G Agúndez; Félix Javier Jiménez-Jiménez Journal: Pharmacogenomics J Date: 2019-12-03 Impact factor: 3.550