Eriberto Bressan1, Michele Stocchero, Ryo Jimbo, Claudio Rosati, Elena Fanti, Cristiano Tomasi, Diego Lops. 1. *Associate Professor, Department of Prosthetic Dentistry, School of Dentistry, University of Padova, Padova, Italy. †PhD Student, Department of Oral and Maxillofacial Surgery and Oral Medicine, Faculty of Odontology, University of Malmö, Malmö, Sweden. ‡Associate Professor, Department of Oral and Maxillofacial Surgery and Oral Medicine, Faculty of Odontology, University of Malmö, Malmö, Sweden. §Laboratory Analyst, Biomolecular Diagnostic S.r.l., Firenze, Italy. ¶Laboratory Director, Biomolecular Diagnostic S.r.l., Firenze, Italy. ‖Associate Professor, Department of Periodontology, Institute of Odontology, The Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden.[LINE SEPARATOR] #Assistant Professor, Department of Prosthodontics, Dental Clinic, School of Dentistry, University of Milan, Milan, Italy.
Abstract
OBJECTIVE: To evaluate in vitro the sealing capability at the prosthetic connection interface of 2 conometric systems. MATERIALS AND METHODS: Two conometric systems with the same design and different material were used, for a total of 24 samples. Each sample was assembled by a tapered abutment and respective coping. In group A, the copings were made of gold, whereas in group B they were made of PEEK. Three μL of mix bacterial suspension (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Fusobacterium nucleatum species) was inoculated into the abutment screw hole, and the coping was inserted on the abutment. Samples were immersed into culture tubes and incubated for 24, 48, and 72 hours into anaerobic conditions. Visual evaluation of turbidity was performed at each time point. Qualitative-quantitative assessment using real-time polymerase chain reaction was performed at 72 hours. Any difference between the groups was checked by means of Fisher exact test. RESULTS: Microbial leakage occurred in both groups, and there was no statistically significant difference between groups. Microbial concentration resulted in a presence inferior to 1 × 10 copies/μL in all positive assemblies. CONCLUSIONS: Because of the low bacterial count, it can be concluded that a minimal bacterial infiltration may be allowed by conometric interfaces for prosthetic connection.
OBJECTIVE: To evaluate in vitro the sealing capability at the prosthetic connection interface of 2 conometric systems. MATERIALS AND METHODS: Two conometric systems with the same design and different material were used, for a total of 24 samples. Each sample was assembled by a tapered abutment and respective coping. In group A, the copings were made of gold, whereas in group B they were made of PEEK. Three μL of mix bacterial suspension (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Fusobacterium nucleatum species) was inoculated into the abutment screw hole, and the coping was inserted on the abutment. Samples were immersed into culture tubes and incubated for 24, 48, and 72 hours into anaerobic conditions. Visual evaluation of turbidity was performed at each time point. Qualitative-quantitative assessment using real-time polymerase chain reaction was performed at 72 hours. Any difference between the groups was checked by means of Fisher exact test. RESULTS: Microbial leakage occurred in both groups, and there was no statistically significant difference between groups. Microbial concentration resulted in a presence inferior to 1 × 10 copies/μL in all positive assemblies. CONCLUSIONS: Because of the low bacterial count, it can be concluded that a minimal bacterial infiltration may be allowed by conometric interfaces for prosthetic connection.
Authors: Peter Gehrke; Philip Hartjen; Ralf Smeets; Martin Gosau; Ulrike Peters; Thomas Beikler; Carsten Fischer; Carolin Stolzer; Jürgen Geis-Gerstorfer; Paul Weigl; Sogand Schäfer Journal: Int J Mol Sci Date: 2021-01-17 Impact factor: 5.923
Authors: Peter Gehrke; Simon Burg; Ulrike Peters; Thomas Beikler; Carsten Fischer; Frank Rupp; Ernst Schweizer; Paul Weigl; Robert Sader; Ralf Smeets; Sogand Schäfer Journal: Clin Oral Investig Date: 2021-08-16 Impact factor: 3.573