Rong Fan1, Tong Pan2, An-Li Zhu3, Mei-Hong Zhang4. 1. Department of Rheumatism and Immunology, Liaocheng People's Hospital, Liaocheng City, Shandong, China. 2. Department of Rheumatism and Immunology, The Second People's Hospital of Liaocheng, Liaocheng City, Shandong, China. 3. Department of Traditional Chinese Medicine, Liaocheng People's Hospital, Liaocheng City, Shandong, China. 4. Department of Urology, Department of orthopaedics, The second people's Hospital of Liaocheng, Liaocheng City, Shandong, China. Electronic address: zhanghongmei192@hotmail.com.
Abstract
BACKGROUND: Naringenin, a bioflavonoid present in various species of citrus fruit, tomatoes and grapes, has been shown to have various pharmacological effects. We evaluated the anti-arthritic potential of naringenin in formaldehyde-induced inflammation and complete Freund's adjuvant (CFA)-induced arthritis. METHODS: For both evaluations, rats were divided into groups of six. Different doses of naringenin (5, 10 and 20mg/kg) were used in the models. Body weight and the arthritic index were assessed. Biochemical and antioxidant parameters were determined. Naringenin dose-dependently reduced joint inflammation, decreasing the joint diameter and inflammatory cell infiltration. RESULTS: Naringenin-treated rats showed an improvement in the synovium redox status (down-regulation of malondialdehyde and glutathione and up-regulation of Catalase (CAT) and superoxide dismutase levels). Naringenin significantly reduced the level of the inflammatory marker TNF-α. Naringenin increased Nrf-2/HO-1 and reduced NF-κB mRNA levels in CFA-treated animal joints. Additionally, naringenin treatment decreased the expression of extracellular matrix degrading enzymes, such as matrix metalloproteinase (MMP-3 and MMP-9), in CFA-induced arthritic rats. CONCLUSIONS: We conclude that naringenin exerts anti-arthritic effects by downregulating NF-κB and activating the Nrf-2/HO-1 pathway.
BACKGROUND:Naringenin, a bioflavonoid present in various species of citrus fruit, tomatoes and grapes, has been shown to have various pharmacological effects. We evaluated the anti-arthritic potential of naringenin in formaldehyde-induced inflammation and complete Freund's adjuvant (CFA)-induced arthritis. METHODS: For both evaluations, rats were divided into groups of six. Different doses of naringenin (5, 10 and 20mg/kg) were used in the models. Body weight and the arthritic index were assessed. Biochemical and antioxidant parameters were determined. Naringenin dose-dependently reduced joint inflammation, decreasing the joint diameter and inflammatory cell infiltration. RESULTS:Naringenin-treated rats showed an improvement in the synovium redox status (down-regulation of malondialdehyde and glutathione and up-regulation of Catalase (CAT) and superoxide dismutase levels). Naringenin significantly reduced the level of the inflammatory marker TNF-α. Naringenin increased Nrf-2/HO-1 and reduced NF-κB mRNA levels in CFA-treated animal joints. Additionally, naringenin treatment decreased the expression of extracellular matrix degrading enzymes, such as matrix metalloproteinase (MMP-3 and MMP-9), in CFA-induced arthritic rats. CONCLUSIONS: We conclude that naringenin exerts anti-arthritic effects by downregulating NF-κB and activating the Nrf-2/HO-1 pathway.
Authors: Mater Hussen Mahnashi; Zeeshan Jabbar; Hafiz Muhammad Irfan; Mulazim Hussain Asim; Muhammad Akram; Ahmed Saif; Mohammed Abdulrahman Alshahrani; Mohammed Ali Alshehri; Saeed Ahmed Asiri Journal: Inflammopharmacology Date: 2021-07-24 Impact factor: 4.473
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