Yao-Jun Zhou1, Hong-Qiong Yang2, Wei Xia1, Li Cui1, Ren-Fang Xu1, Hao Lu1, Zhong Xue1, Bo Zhang1, Zi-Nong Tian1, Yun-Jie Cao1, Zhao-Yu Xing1, Shuai Yin1, Xiao-Zhou He3. 1. Department of Surgical Urology, the Third Affiliated Hospital of Soochow University, Changzhou 213003, China. 2. Department of Geriatric Medicine, the Third Affiliated Hospital of Soochow University, Changzhou 213003, China. 3. Department of Surgical Urology, the Third Affiliated Hospital of Soochow University, Changzhou 213003, China. Electronic address: Xiaozhou_He@163.com.
Abstract
AIMS: MicroRNA served as inhibitor for gene expression in various cancers. This study aimed to investigate the role of miR-605 and EN2 in prostate cancer (PCa). MATERIALS AND METHODS: In this research, the expression of miR-605 and EN2 protein in PCa tissues and cells were determined by qRT-PCR and western blot, respectively. The cell proliferation was measured by Cell Counting Kit-8 (CCK-8) and the tumor cell invasion assay was accomplished with transwell system. Flow cytometry was used to analyze the cell cycle. The endogenous expression of miR-605 and EN2 was modulated by recombinant plasmids and cell transfection. Dual luciferase reporter assay was performed to determine the interaction between miR-605 and EN2 in PCa cells. KEY FINDINGS: The expression of miR-605 was lower in PCa tissue and cells than that in normal tissues and cells, while the expression of EN2 was just the opposite. Down-regulation of the EN2 by siRNA inhibited the proliferation and invasion of PC3 cells, and the cell cycle was arrested in G0/G1 phase. EN2 regulated the expression of E-cadherin and Vimentin through Snail and EN2 regulated the cell cycle and cell proliferation via PI3K/AKT pathway. MiR-605 inhibited the proliferation and invasion of PCa cells through targeting EN2. SIGNIFICANCE: EN2 is negatively regulated by miR-605, and down-regulation of miR-605 promotes the proliferation and invasion of PCa cells by up-regulating EN2, which leads to PCa development and progression.
AIMS: MicroRNA served as inhibitor for gene expression in various cancers. This study aimed to investigate the role of miR-605 and EN2 in prostate cancer (PCa). MATERIALS AND METHODS: In this research, the expression of miR-605 and EN2 protein in PCa tissues and cells were determined by qRT-PCR and western blot, respectively. The cell proliferation was measured by Cell Counting Kit-8 (CCK-8) and the tumor cell invasion assay was accomplished with transwell system. Flow cytometry was used to analyze the cell cycle. The endogenous expression of miR-605 and EN2 was modulated by recombinant plasmids and cell transfection. Dual luciferase reporter assay was performed to determine the interaction between miR-605 and EN2 in PCa cells. KEY FINDINGS: The expression of miR-605 was lower in PCa tissue and cells than that in normal tissues and cells, while the expression of EN2 was just the opposite. Down-regulation of the EN2 by siRNA inhibited the proliferation and invasion of PC3 cells, and the cell cycle was arrested in G0/G1 phase. EN2 regulated the expression of E-cadherin and Vimentin through Snail and EN2 regulated the cell cycle and cell proliferation via PI3K/AKT pathway. MiR-605 inhibited the proliferation and invasion of PCa cells through targeting EN2. SIGNIFICANCE: EN2 is negatively regulated by miR-605, and down-regulation of miR-605 promotes the proliferation and invasion of PCa cells by up-regulating EN2, which leads to PCa development and progression.
Authors: Alexander B Coley; Ashlyn N Stahly; Mohan V Kasukurthi; Addison A Barchie; Sam B Hutcheson; Dominika Houserova; Yulong Huang; Brianna C Watters; Valeria M King; Meghan A Dean; Justin T Roberts; Jeffrey D DeMeis; Krisha V Amin; Cameron H McInnis; Noel L Godang; Ryan M Wright; David F Haider; Neha B Piracha; Cana L Brown; Zohaib M Ijaz; Shengyu Li; Yaguang Xi; Oliver G McDonald; Jingshan Huang; Glen M Borchert Journal: Cells Date: 2022-04-12 Impact factor: 7.666