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Literature DB >> 28941028

Luciferases with Tunable Emission Wavelengths.

Julien Hiblot^1,2, Qiuliyang Yu^1,2, Marina D B Sabbadini¹, Luc Reymond¹, Lin Xue^1,2, Alberto Schena¹, Olivier Sallin¹, Nicholas Hill¹, Rudolf Griss¹, Kai Johnsson^1,2.

Abstract

We introduce luciferases whose emission maxima can be tuned to different wavelengths by chemical labeling. The luciferases are chimeras of NanoLuc with either SNAP-tag or HaloTag7. Labeling of the self-labeling tag with a fluorophore shifts the emission maximum of NanoLuc to that of the fluorophore. Luciferases with tunable colors have applications as reporter genes, for the construction of biosensors and in bioimaging.

Keywords: bioluminescent imaging; luciferase; self-labeling protein; sensors; therapeutic drug monitoring

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Substances：

Year: 2017 PMID： 28941028 DOI： 10.1002/anie.201708277

Source DB: PubMed Journal: Angew Chem Int Ed Engl ISSN： 1433-7851 Impact factor: 15.336

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Cited

15 in total

1. Visible-to-NIR-Light Activated Release: From Small Molecules to Nanomaterials.

Authors: Roy Weinstain; Tomáš Slanina; Dnyaneshwar Kand; Petr Klán
Journal: Chem Rev Date: 2020-10-30 Impact factor: 60.622

2. Building Biological Flashlights: Orthogonal Luciferases and Luciferins for in Vivo Imaging.

Authors: Sierra J Williams; Jennifer A Prescher
Journal: Acc Chem Res Date: 2019-10-08 Impact factor: 22.384

Review 3. Development and Applications of Bioluminescent and Chemiluminescent Reporters and Biosensors.

Authors: Hsien-Wei Yeh; Hui-Wang Ai
Journal: Annu Rev Anal Chem (Palo Alto Calif) Date: 2019-02-20 Impact factor: 10.745

4. Engineered Amber-Emitting Nano Luciferase and Its Use for Immunobioluminescence Imaging In Vivo.

Authors: Ying Xiong; Yiyu Zhang; Zefan Li; Md Shamim Reza; Xinyu Li; Xiaodong Tian; Hui-Wang Ai
Journal: J Am Chem Soc Date: 2022-08-01 Impact factor: 16.383

5. Rapid Quantification of C. difficile Glutamate Dehydrogenase and Toxin B (TcdB) with a NanoBiT Split-Luciferase Assay.

Authors: Hope Adamson; Modupe O Ajayi; Kate E Gilroy; Michael J McPherson; Darren C Tomlinson; Lars J C Jeuken
Journal: Anal Chem Date: 2022-05-28 Impact factor: 8.008

Luciferases with Tunable Emission Wavelengths.

1. Visible-to-NIR-Light Activated Release: From Small Molecules to Nanomaterials.

2. Building Biological Flashlights: Orthogonal Luciferases and Luciferins for in Vivo Imaging.

Review 3. Development and Applications of Bioluminescent and Chemiluminescent Reporters and Biosensors.

4. Engineered Amber-Emitting Nano Luciferase and Its Use for Immunobioluminescence Imaging In Vivo.

5. Rapid Quantification of C. difficile Glutamate Dehydrogenase and Toxin B (TcdB) with a NanoBiT Split-Luciferase Assay.

Review 6. Seeing (and Using) the Light: Recent Developments in Bioluminescence Technology.

7. Ratiometric Bioluminescent Sensor Proteins Based on Intramolecular Split Luciferase Complementation.

Review 8. Advanced Bioluminescence System for In Vivo Imaging with Brighter and Red-Shifted Light Emission.

9. Bioluminescent Antibodies through Photoconjugation of Protein G-Luciferase Fusion Proteins.

10. Anti-tumor Synergistic Effect of a Dual Cancer-Specific Recombinant Adenovirus and Paclitaxel on Breast Cancer.