Modeling of ultrasound-assisted extraction, characterization and in vitro pharmacological potential of polysaccharides from Vaccinium arctostaphylos L.

In the study, Box-Behnken design was used to optimized the operational parameters involved in ultrasound-assisted extraction (UAE) of Vaccinium arctostaphylos polysaccharide (VAP) including ultrasound power, extraction temperature and time and water to raw material ratio. The highest extraction polysaccharide was obtained at 100W, 19.12min, 79.32C and 20 (ml water/g powdered Vaccinium arctostaphylos). Under these condition, total anthocyanin, total phenolic contents and total flavonoid were 10.16mlg/g, 0.43mlg/g and 0.58mlg/g, respectably. Also, structure of polysaccharide was identified by GC-Mass Fourie transform-infrared spectroscopy (FT-IR). Then, we demonstrated that VAPs had significantly scavenging activities against 2,2-diphenyl-1-picrylhydrazyl and hydroxyl radicals in vitro. The effect of degradation of DPPH was increased by increasing the concentration of polysaccharide up to 15mg/ml. The anti-microbial activity of the resulted polysaccharide with highest concentration (15mg/ml) was the following in order of inhibition zone diameter: B. panis (9.89mm)>S. aureus (9.12mm)>Sh. dysenteriae (7.45mm)>E. coli (6.38mm). VAPs can thus be used as anexcellent antioxidant and antimicrobial ingredient in food and medicinal preparations.