Background: A single subtype of canine influenza virus (CIV), A(H3N8), was circulating in the United States until a new subtype, A(H3N2), was detected in Illinois in spring 2015. Since then, this CIV has caused thousands of infections in dogs in multiple states. Methods: In this study, genetic and antigenic properties of the new CIV were evaluated. In addition, structural and glycan array binding features of the recombinant hemagglutinin were determined. Replication kinetics in human airway cells and pathogenesis and transmissibility in animal models were also assessed. Results: A(H3N2) CIVs maintained molecular and antigenic features related to low pathogenicity avian influenza A(H3N2) viruses and were distinct from A(H3N8) CIVs. The structural and glycan array binding profile confirmed these findings and revealed avian-like receptor-binding specificity. While replication kinetics in human airway epithelial cells was on par with that of seasonal influenza viruses, mild-to-moderate disease was observed in infected mice and ferrets, and the virus was inefficiently transmitted among cohoused ferrets. Conclusions: Further adaptation is needed for A(H3N2) CIVs to present a likely threat to humans. However, the potential for coinfection of dogs and possible reassortment of human and other animal influenza A viruses presents an ongoing risk to public health. Published by Oxford University Press for the Infectious Diseases Society of America 2017. This work is written by (a) US Government employee(s) and is in the public domain in the US.
Background: A single subtype of canineinfluenza virus (CIV), A(H3N8), was circulating in the United States until a new subtype, A(H3N2), was detected in Illinois in spring 2015. Since then, this CIV has caused thousands of infections in dogs in multiple states. Methods: In this study, genetic and antigenic properties of the new CIV were evaluated. In addition, structural and glycan array binding features of the recombinant hemagglutinin were determined. Replication kinetics in human airway cells and pathogenesis and transmissibility in animal models were also assessed. Results: A(H3N2) CIVs maintained molecular and antigenic features related to low pathogenicity avian influenza A(H3N2) viruses and were distinct from A(H3N8) CIVs. The structural and glycan array binding profile confirmed these findings and revealed avian-like receptor-binding specificity. While replication kinetics in human airway epithelial cells was on par with that of seasonal influenza viruses, mild-to-moderate disease was observed in infected mice and ferrets, and the virus was inefficiently transmitted among cohoused ferrets. Conclusions: Further adaptation is needed for A(H3N2) CIVs to present a likely threat to humans. However, the potential for coinfection of dogs and possible reassortment of human and other animal influenza A viruses presents an ongoing risk to public health. Published by Oxford University Press for the Infectious Diseases Society of America 2017. This work is written by (a) US Government employee(s) and is in the public domain in the US.
Authors: Taronna R Maines; Li-Mei Chen; Yumiko Matsuoka; Hualan Chen; Thomas Rowe; Juan Ortin; Ana Falcón; Tran Hien Nguyen; Le Quynh Mai; Endang R Sedyaningsih; Syahrial Harun; Terrence M Tumpey; Ruben O Donis; Nancy J Cox; Kanta Subbarao; Jacqueline M Katz Journal: Proc Natl Acad Sci U S A Date: 2006-07-31 Impact factor: 11.205
Authors: H Moon; M Hong; J K Kim; B Seon; W Na; S J Park; D J An; H Y Jeoung; D J Kim; J M Kim; S H Kim; R J Webby; R G Webster; B K Kang; D Song Journal: Epidemiol Infect Date: 2014-06-30 Impact factor: 4.434
Authors: Luis Martinez-Sobrido; Pilar Blanco-Lobo; Laura Rodriguez; Theresa Fitzgerald; Hanyuan Zhang; Phuong Nguyen; Christopher S Anderson; Jeanne Holden-Wiltse; Sanjukta Bandyopadhyay; Aitor Nogales; Marta L DeDiego; Brian R Wasik; Benjamin L Miller; Carole Henry; Patrick C Wilson; Mark Y Sangster; John J Treanor; David J Topham; Lauren Byrd-Leotis; David A Steinhauer; Richard D Cummings; Jasmina M Luczo; Stephen M Tompkins; Kaori Sakamoto; Cheryl A Jones; John Steel; Anice C Lowen; Shamika Danzy; Hui Tao; Ashley L Fink; Sabra L Klein; Nicholas Wohlgemuth; Katherine J Fenstermacher; Farah El Najjar; Andrew Pekosz; Lauren Sauer; Mitra K Lewis; Kathryn Shaw-Saliba; Richard E Rothman; Zhen-Ying Liu; Kuan-Fu Chen; Colin R Parrish; Ian E H Voorhees; Yoshihiro Kawaoka; Gabriele Neumann; Shiho Chiba; Shufang Fan; Masato Hatta; Huihui Kong; Gongxun Zhong; Guojun Wang; Melissa B Uccellini; Adolfo García-Sastre; Daniel R Perez; Lucas M Ferreri; Sander Herfst; Mathilde Richard; Ron Fouchier; David Burke; David Pattinson; Derek J Smith; Victoria Meliopoulos; Pamela Freiden; Brandi Livingston; Bridgett Sharp; Sean Cherry; Juan Carlos Dib; Guohua Yang; Charles J Russell; Subrata Barman; Richard J Webby; Scott Krauss; Angela Danner; Karlie Woodard; Malik Peiris; R A P M Perera; M C W Chan; Elena A Govorkova; Bindumadhav M Marathe; Philippe N Q Pascua; Gavin Smith; Yao-Tsun Li; Paul G Thomas; Stacey Schultz-Cherry Journal: PLoS Pathog Date: 2020-04-14 Impact factor: 6.823