Literature DB >> 28933823

Two-Color 810 nm STED Nanoscopy of Living Cells with Endogenous SNAP-Tagged Fusion Proteins.

Alexey N Butkevich1, Haisen Ta1, Michael Ratz1, Stefan Stoldt1, Stefan Jakobs1,2, Vladimir N Belov1, Stefan W Hell1.   

Abstract

A 810 nm STED nanoscopy setup and an appropriate combination of two fluorescent dyes (Si-rhodamine 680SiR and carbopyronine 610CP) have been developed for near-IR live-cell super-resolution imaging. Vimentin endogenously tagged using the CRISPR/Cas9 approach with the SNAP tag, together with a noncovalent tubulin label, provided reliable and cell-to-cell reproducible dual-color confocal and STED imaging of the cytoskeleton in living cells.

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Year:  2017        PMID： 28933823     DOI： 10.1021/acschembio.7b00616

Source DB:  PubMed          Journal:  ACS Chem Biol        ISSN： 1554-8929            Impact factor:   5.100


  21 in total

Review 1.  Fluorescence microscopy applied to intracellular transport by microtubule motors.

Authors:  Divya Pathak; Shreyasi Thakur; Roop Mallik
Journal:  J Biosci       Date:  2018-07       Impact factor: 1.826

2.  isoSTED microscopy with water-immersion lenses and background reduction.

Authors:  René Siegmund; Frank Werner; Stefan Jakobs; Claudia Geisler; Alexander Egner
Journal:  Biophys J       Date:  2021-07-09       Impact factor: 3.699

3.  Fluorescent dyes and probes for super-resolution microscopy of microtubules and tracheoles in living cells and tissues.

Authors:  Gražvydas Lukinavičius; Gyuzel Y Mitronova; Sebastian Schnorrenberg; Alexey N Butkevich; Hannah Barthel; Vladimir N Belov; Stefan W Hell
Journal:  Chem Sci       Date:  2018-02-26       Impact factor: 9.825

4.  Live-cell labeling of endogenous proteins with nanometer precision by transduced nanobodies.

Authors:  A Klein; S Hank; A Raulf; E F Joest; F Tissen; M Heilemann; R Wieneke; R Tampé
Journal:  Chem Sci       Date:  2018-08-20       Impact factor: 9.825

5.  Near-infrared STED nanoscopy with an engineered bacterial phytochrome.

Authors:  Maria Kamper; Haisen Ta; Nickels A Jensen; Stefan W Hell; Stefan Jakobs
Journal:  Nat Commun       Date:  2018-11-12       Impact factor: 14.919

6.  Newly synthesized claudins but not occludin are added to the basal side of the tight junction.

Authors:  Christina M Van Itallie; Karin Fredriksson Lidman; Amber Jean Tietgens; James Melvin Anderson
Journal:  Mol Biol Cell       Date:  2019-04-03       Impact factor: 4.138

7.  Triarylmethane Fluorophores Resistant to Oxidative Photobluing.

Authors:  Alexey N Butkevich; Mariano L Bossi; Gražvydas Lukinavičius; Stefan W Hell
Journal:  J Am Chem Soc       Date:  2019-01-02       Impact factor: 15.419

8.  A new approach to silicon rhodamines by Suzuki-Miyaura coupling - scope and limitations.

Authors:  Thines Kanagasundaram; Antje Timmermann; Carsten S Kramer; Klaus Kopka
Journal:  Beilstein J Org Chem       Date:  2019-10-29       Impact factor: 2.883

Review 9.  Near-infrared fluorescent probes: a next-generation tool for protein-labeling applications.

Authors:  Shahi Imam Reja; Masafumi Minoshima; Yuichiro Hori; Kazuya Kikuchi
Journal:  Chem Sci       Date:  2020-10-23       Impact factor: 9.825

10.  Robust nanoscopy of a synaptic protein in living mice by organic-fluorophore labeling.

Authors:  Jennifer-Magdalena Masch; Heinz Steffens; Joachim Fischer; Johann Engelhardt; Jasmine Hubrich; Jan Keller-Findeisen; Elisa D'Este; Nicolai T Urban; Seth G N Grant; Steffen J Sahl; Dirk Kamin; Stefan W Hell
Journal:  Proc Natl Acad Sci U S A       Date:  2018-08-06       Impact factor: 11.205
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