Hun Park1, Uhram Song2. 1. a Institute for Legal Studies, Yonsei University , Seoul , South Korea. 2. b Department of Biology and Research Institute for Basic Sciences , Jeju National University , Jeju , South Korea.
Abstract
Although Azolla species are among the most promising plants for use in phytoremediation, more studies on their growth and nitrogen (N) uptake along the N gradients of growing media are required. In this study, N concentration-dependent growth in growing media and phosphorus (P) and N accumulation by Azolla japonica were studied by estimating direct N uptake from media by molybdenum-iron proteins. The doubling time of A. japonica was less than a week, regardless of the N concentration (0, 5, and 25 mg N/L) present in the growth media, indicating that this plant is suitable for remediation. Plants showed a high uptake of P, probably via plant-bacteria symbiosis, indicating their potential for effective P remediation. A. japonica also showed more than 4% N content regardless of the treatment and accumulated more than 40 mg of N per microcosm in 3 weeks. iron and molybdenum levels in plants were strongly associated with N fixation, and N uptake from media was estimated to be more than 25 mg per microcosm in 3 weeks, indicating that A. japonica has N remediation potential. As A. japonica is a rapidly growing plant, capable of efficient P and N remediation, it has great potential for use in phytoremediation of nutrient-enriched waters such as agricultural or urban wastewater and eutrophicated aquatic ecosystems.
Although Azolla species are among the most promising plants for use in phytoremediation, more studies on their growth and n class="Chemical">nitrogen (N) uptake alonpan>g the N gradienpan>ts of growinpan>g media are required. Inpan> this study, N conpan>cenpan>trationpan>-depenpan>denpan>t growth inpan> growinpan>g media anpan>d pan> class="Chemical">phosphorus (P) and N accumulation by Azolla japonica were studied by estimating direct N uptake from media by molybdenum-iron proteins. The doubling time of A. japonica was less than a week, regardless of the N concentration (0, 5, and 25 mg N/L) present in the growth media, indicating that this plant is suitable for remediation. Plants showed a high uptake of P, probably via plant-bacteria symbiosis, indicating their potential for effective P remediation. A. japonica also showed more than 4% N content regardless of the treatment and accumulated more than 40 mg of N per microcosm in 3 weeks. iron and molybdenum levels in plants were strongly associated with N fixation, and N uptake from media was estimated to be more than 25 mg per microcosm in 3 weeks, indicating that A. japonica has N remediation potential. As A. japonica is a rapidly growing plant, capable of efficient P and N remediation, it has great potential for use in phytoremediation of nutrient-enriched waters such as agricultural or urban wastewater and eutrophicated aquatic ecosystems.
Authors: Federico Brilli; K G Srikanta Dani; Stefania Pasqualini; Alma Costarelli; Sara Cannavò; Francesco Paolocci; Graziella Chini Zittelli; Gianmarco Mugnai; Rita Baraldi; Francesco Loreto Journal: Physiol Plant Date: 2022-01 Impact factor: 5.081