Elizangela Partata Zuza1,2, Valdir Gouveia Garcia3, Letícia Helena Theodoro3, Edilson Ervolino4, Luiz Fernando Veloso Favero5, Mariéllen Longo3, Fernando Salimon Ribeiro5, Alex Tadeu Martins5, Luís Carlos Spolidorio6, José Antônio Sampaio Zuanon6, Benedicto Egbert Corrêa de Toledo7, Juliana Rico Pires5. 1. Department of Periodontology, School of Dentistry, Fluminense Federal University (UFF), Nova Friburgo, Rio de Janeiro, Brazil. elizangelazuza@yahoo.com.br. 2. Elizangela Partata Zuza, Instituto de Saúde de Nova Friburgo (ISNF)-UFF, Rua Dr. Silvio Henrique Braune, 22. Cep: 28625-650. Nova Friburgo, Rio de Janeiro, Brazil. elizangelazuza@yahoo.com.br. 3. Department of Surgery and Integrated Clinic, School of Dentistry, São Paulo State University (Unesp), Araçatuba, São Paulo, Brazil. 4. Department of Basic Science, School of Dentistry, São Paulo State University (Unesp), Araçatuba, São Paulo, Brazil. 5. Department of Dentistry, School of Dentistry, Educational Foundation of Barretos (Unifeb), Barretos, São Paulo, Brazil. 6. Department of Physiology and Pathology, School of Dentistry, São Paulo State University (Unesp), Araraquara, São Paulo, Brazil. 7. Department of Diagnosis and Surgery, School of Dentistry, São Paulo State University (Unesp), Araraquara, Brazil.
Abstract
OBJECTIVES: This study assessed the influence of obesity on the progression of ligature-induced periodontitis in rats. MATERIALS AND METHODS: Forty-eight adult Wistar rats were randomly divided into two groups: the HL group (n = 24) was fed high-fat animal food to induce obesity, and the NL group (n = 24) was fed normolipidic animal food. Obesity was induced within a period of 120 days, and the induction of experimental periodontitis (EP) was subsequently performed for 30 days. The animals were euthanized after 7, 15, and 30 days, and the jaws were removed for histopathological, histometric, and immunohistochemical analyses. Tartrate-resistant acid phosphatase (TRAP), receptor activator of nuclear factor kappa beta ligand (RANKL), and osteoprotegerin (OPG) were analyzed via immunolabeling. RESULTS: Histological findings indicated that the inflammation was more extensive and lasted longer in the HL⁄EP; however, advanced destruction also occurred in the NL/EP. Greater bone loss was verified in the HL/EP group (2.28 ± 0.35) in the period of 7 days than in the NL/EP group (1.2 ± 0.29). High immunolabeling was identified in the HL/EP group in the initial periods for RANKL and TRAP, whereas the NL⁄EP group presented with moderate immunolabeling for both factors. The HL/EP and NL/EP groups showed low immunolabeling for OPG. CONCLUSIONS: Obesity induced by a high-fat diet influenced alveolar bone metabolism when associated with experimental periodontitis and caused a more severe local inflammatory response and alveolar bone loss. CLINICAL RELEVANCE: Obesity is related to greater alveolar bone loss and an accentuated local inflammatory response, which may be reflected in the clinical severity of periodontitis and dental loss.
OBJECTIVES: This study assessed the influence of obesity on the progression of ligature-induced periodontitis in rats. MATERIALS AND METHODS: Forty-eight adult Wistar rats were randomly divided into two groups: the HL group (n = 24) was fed high-fat animal food to induce obesity, and the NL group (n = 24) was fed normolipidic animal food. Obesity was induced within a period of 120 days, and the induction of experimental periodontitis (EP) was subsequently performed for 30 days. The animals were euthanized after 7, 15, and 30 days, and the jaws were removed for histopathological, histometric, and immunohistochemical analyses. Tartrate-resistant acid phosphatase (TRAP), receptor activator of nuclear factor kappa beta ligand (RANKL), and osteoprotegerin (OPG) were analyzed via immunolabeling. RESULTS: Histological findings indicated that the inflammation was more extensive and lasted longer in the HL⁄EP; however, advanced destruction also occurred in the NL/EP. Greater bone loss was verified in the HL/EP group (2.28 ± 0.35) in the period of 7 days than in the NL/EP group (1.2 ± 0.29). High immunolabeling was identified in the HL/EP group in the initial periods for RANKL and TRAP, whereas the NL⁄EP group presented with moderate immunolabeling for both factors. The HL/EP and NL/EP groups showed low immunolabeling for OPG. CONCLUSIONS:Obesity induced by a high-fat diet influenced alveolar bone metabolism when associated with experimental periodontitis and caused a more severe local inflammatory response and alveolar bone loss. CLINICAL RELEVANCE: Obesity is related to greater alveolar bone loss and an accentuated local inflammatory response, which may be reflected in the clinical severity of periodontitis and dental loss.
Entities:
Keywords:
Alveolar bone loss; Body weight; Inflammation; Obesity; Periodontitis; Rats
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