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Literature DB >> 2892830

Introduction of the Escherichia coli gdhA gene into Rhizobium phaseoli: effect on nitrogen fixation.

Abstract

Rhizobium phaseoli lacks glutamate dehydrogenase (GDH) and assimilates ammonium by the glutamine synthetase-glutamate synthase pathway. A strain of R. phaseoli harboring the Escherichia coli GDH structural gene (gdhA) was constructed. GDH activity was expressed in R. phaseoli in the free-living state and in symbiosis. Nodules with bacteroids that expressed GDH activity had severe impairment of nitrogen fixation. Also, R. phaseoli cells that lost GDH activity and assimilated ammonium by the glutamine synthetase-glutamate synthase pathway preferentially nodulated Phaseolus vulgaris.

Entities: Chemical Species

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Year: 1988 PMID： 2892830 PMCID： PMC210752 DOI： 10.1128/jb.170.2.985-988.1988

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

20 in total

1. Enzymes of ammonia assimilation in Rhizobium leguminosarum bacteroids.

Authors: W G Kurz; D A Rokosh; T A LaRue
Journal: Can J Microbiol Date: 1975-07 Impact factor: 2.419

2. Induction of glutamate synthase during nodule development in lupin.

Authors: J G Robertson; M P Warburton; K J Farnden
Journal: FEBS Lett Date: 1975-07-15 Impact factor: 4.124

3. Control of ammonium assimilation in Rhizobium 32H1.

Authors: R A Ludwig
Journal: J Bacteriol Date: 1978-07 Impact factor: 3.490

4. Mutations in Rhizobium phaseoli that lead to arrested development of infection threads.

Authors: K D Noel; K A Vandenbosch; B Kulpaca
Journal: J Bacteriol Date: 1986-12 Impact factor: 3.490

5. R factor transfer in Rhizobium leguminosarum.

Authors: J E Beringer
Journal: J Gen Microbiol Date: 1974-09

6. A complementation analysis of the restriction and modification of DNA in Escherichia coli.

Authors: H W Boyer; D Roulland-Dussoix
Journal: J Mol Biol Date: 1969-05-14 Impact factor: 5.469

7. Inhibition of homocysteine sulfonamide of glutamate synthase purified from Saccharomyces cerevisiae.

Authors: D S Masters; A Meister
Journal: J Biol Chem Date: 1982-08-10 Impact factor: 5.157

8. Coregulation of oxidized nicotinamide adenine dinucleotide (phosphate) transhydrogenase and glutamate dehydrogenase activities in enteric bacteria during nitrogen limitation.

Authors: A Liang; R L Houghton
Journal: J Bacteriol Date: 1981-06 Impact factor: 3.490

5. Expression of the Escherichia coli glutamate dehydrogenase gene in the cyanobacterium Synechococcus PCC6301 causes ammonium tolerance.

Authors: D A Lightfoot; A J Baron; J C Wootton
Journal: Plant Mol Biol Date: 1988-05 Impact factor: 4.076

5 in total

Introduction of the Escherichia coli gdhA gene into Rhizobium phaseoli: effect on nitrogen fixation.

1. Enzymes of ammonia assimilation in Rhizobium leguminosarum bacteroids.

2. Induction of glutamate synthase during nodule development in lupin.

3. Control of ammonium assimilation in Rhizobium 32H1.

4. Mutations in Rhizobium phaseoli that lead to arrested development of infection threads.

5. R factor transfer in Rhizobium leguminosarum.

6. A complementation analysis of the restriction and modification of DNA in Escherichia coli.

7. Inhibition of homocysteine sulfonamide of glutamate synthase purified from Saccharomyces cerevisiae.

8. Coregulation of oxidized nicotinamide adenine dinucleotide (phosphate) transhydrogenase and glutamate dehydrogenase activities in enteric bacteria during nitrogen limitation.

9. Ammonium assimilation in Rhizobium phaseoli by the glutamine synthetase-glutamate synthase pathway.

10. Ammonium assimilation in Rhizobium meliloti.

1. Key role of bacterial NH(4)(+) metabolism in Rhizobium-plant symbiosis.

2. Glutamate is involved in acid stress response in Bradyrhizobium sp. SEMIA 6144 (Arachis hypogaea L.) microsymbiont.

3. Pyruvate carboxylase from Rhizobium etli: mutant characterization, nucleotide sequence, and physiological role.

4. Ammonium assimilation in Rhizobium phaseoli by the glutamine synthetase-glutamate synthase pathway.

5. Expression of the Escherichia coli glutamate dehydrogenase gene in the cyanobacterium Synechococcus PCC6301 causes ammonium tolerance.