Literature DB >> 28927972

Propidium ion enters viable cells with high membrane potential during live-dead staining.

Christian Kirchhoff1, Heribert Cypionka2.   

Abstract

Live-dead staining with propidium iodide can give erroneous results for bacteria showing high membrane potentials. We observed uptake of propidium ions across intact cell membranes for Dinoroseobacter shibae and Bacillus subtilis. Apparently, a high membrane potential facilitates breakthrough of the double-charged propidium ion and can mark viable cells as dead.
Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Bacillus subtilis; DiOC(2)(3); Dinoroseobacter shibae; Fluorescence microscopy; Propidium iodide; Sybr Green I

Mesh：

Substances：

Year:  2017        PMID： 28927972     DOI： 10.1016/j.mimet.2017.09.011

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  16 in total

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