Literature DB >> 2892531

Folate binding and transport by rat kidney brush-border membrane vesicles.

S D Bhandari1, S K Joshi, K E McMartin.   

Abstract

[3H]Pteroylglutamic acid (PteGlu) uptake was studied using brush-border membrane vesicles isolated from rat kidney. Results on the uptake of [3H]PteGlu by brush-border membrane vesicles incubated in media of increasing osmolarities demonstrated that uptake was contributed by two components, intravesicular transport and membrane binding. Both the components of the uptake exhibited similar pH dependence, with maxima at pH 5.6, and were found to be saturable mechanisms with Km values of 6.7.10(-7) and 11.2.10(-7) M, respectively. These studies show that PteGlu is transported by isolated rat kidney brush-border membrane vesicles in a manner consistent with a saturable system and that a binding component may be functionally associated with this.

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Year:  1988        PMID： 2892531     DOI： 10.1016/0005-2736(88)90243-x

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  7 in total

Review 1.  Mechanisms of membrane transport of folates into cells and across epithelia.

Authors:  Rongbao Zhao; Ndeye Diop-Bove; Michele Visentin; I David Goldman
Journal:  Annu Rev Nutr       Date:  2011-08-21       Impact factor: 11.848

2.  Kinetic characteristics of folate binding to rat renal brush border membrane in chronic alcoholism.

Authors:  Abid Hamid; Jyotdeep Kaur
Journal:  Mol Cell Biochem       Date:  2005-12       Impact factor: 3.396

Review 3.  Membrane transporters and folate homeostasis: intestinal absorption and transport into systemic compartments and tissues.

Authors:  Rongbao Zhao; Larry H Matherly; I David Goldman
Journal:  Expert Rev Mol Med       Date:  2009-01-28       Impact factor: 5.600

4.  Riboflavin uptake by rat small intestinal brush border membrane vesicles: a dual mechanism involving specific membrane binding.

Authors:  D Casirola; G Gastaldi; G Ferrari; S Kasai; G Rindi
Journal:  J Membr Biol       Date:  1993-09       Impact factor: 1.843

5.  Ligand-induced conformation change in folate-binding protein.

Authors:  N C Kaarsholm; A M Kolstrup; S E Danielsen; J Holm; S I Hansen
Journal:  Biochem J       Date:  1993-06-15       Impact factor: 3.857

6.  Reduced expression of folate transporters in kidney of a rat model of folate oversupplementation.

Authors:  Shilpa Thakur; Som Dev Thakur; Nissar Ahmad Wani; Jyotdeep Kaur
Journal:  Genes Nutr       Date:  2013-12-04       Impact factor: 5.523

7.  N-linked glycosylation and its impact on the electrophoretic mobility and function of the human proton-coupled folate transporter (HsPCFT).

Authors:  Ersin Selcuk Unal; Rongbao Zhao; Andong Qiu; I David Goldman
Journal:  Biochim Biophys Acta       Date:  2008-03-20
  7 in total

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