Literature DB >> 2892401

Detection of a NotI polymorphism with the pmetH probe by pulsed-field gel electrophoresis.

C Julier1, R White.   

Abstract

The pmetH probe, tightly linked to the locus for cystic fibrosis (CF), detected a NotI polymorphism with allele sizes of 1,000 and 550 kb that could be separated by pulsed-field gel electrophoresis. Both alleles were found on chromosomes bearing either the normal or the CF allele. Preliminary data showed that this polymorphism was not in strong linkage disequilibrium with the CF locus. Preliminary large-scale restriction mapping of the fragment showed that the NotI polymorphic site is 200-370 kb distant from the MET locus; thus it defines a new polymorphic locus in the CF region. This locus is of potential interest for studying CF families that show recombinants between CF and its tightly linked probes, in order to increase the precision of the genetic map of the region.

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Year:  1988        PMID： 2892401      PMCID： PMC1715310     

Source DB:  PubMed          Journal:  Am J Hum Genet        ISSN： 0002-9297            Impact factor:   11.025


  13 in total

1.  Linkage of cystic fibrosis to two tightly linked DNA markers: joint report from a collaborative study.

Authors:  A Beaudet; A Bowcock; M Buchwald; L Cavalli-Sforza; M Farrall; M C King; K Klinger; J M Lalouel; G Lathrop; S Naylor
Journal:  Am J Hum Genet       Date:  1986-12       Impact factor: 11.025

2.  Regional mapping of six cloned DNA sequences on human chromosome 7.

Authors:  I Bartels; K H Grzeschik; D N Cooper; J Schmidtke
Journal:  Am J Hum Genet       Date:  1986-03       Impact factor: 11.025

3.  Report of the Committee on Human Gene Mapping by Recombinant DNA Techniques.

Authors:  H F Willard; M H Skolnick; P L Pearson; J L Mandel
Journal:  Cytogenet Cell Genet       Date:  1985

4.  Isolation of a further anonymous informative DNA sequence from chromosome seven closely linked to cystic fibrosis.

Authors:  P J Scambler; B J Wainwright; E Watson; G Bates; G Bell; R Williamson; M Farrall
Journal:  Nucleic Acids Res       Date:  1986-03-11       Impact factor: 16.971

5.  A candidate for the cystic fibrosis locus isolated by selection for methylation-free islands.

Authors:  X Estivill; M Farrall; P J Scambler; G M Bell; K M Hawley; N J Lench; G P Bates; H C Kruyer; P A Frederick; P Stanier
Journal:  Nature       Date:  1987 Apr 30-May 6       Impact factor: 49.962

6.  Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis.

Authors:  G F Carle; M V Olson
Journal:  Nucleic Acids Res       Date:  1984-07-25       Impact factor: 16.971

7.  Molecular cloning of a new transforming gene from a chemically transformed human cell line.

Authors:  C S Cooper; M Park; D G Blair; M A Tainsky; K Huebner; C M Croce; G F Vande Woude
Journal:  Nature       Date:  1984 Sep 6-11       Impact factor: 49.962

8.  Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.

Authors:  D C Schwartz; C R Cantor
Journal:  Cell       Date:  1984-05       Impact factor: 41.582

9.  A closely linked genetic marker for cystic fibrosis.

Authors:  R White; S Woodward; M Leppert; P O'Connell; M Hoff; J Herbst; J M Lalouel; M Dean; G Vande Woude
Journal:  Nature       Date:  1985 Nov 28-Dec 4       Impact factor: 49.962

10.  Localization of cystic fibrosis locus to human chromosome 7cen-q22.

Authors:  B J Wainwright; P J Scambler; J Schmidtke; E A Watson; H Y Law; M Farrall; H J Cooke; H Eiberg; R Williamson
Journal:  Nature       Date:  1985 Nov 28-Dec 4       Impact factor: 49.962
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  4 in total

1.  A 12 megabase restriction map at the cystic fibrosis locus.

Authors:  T R Fulton; A M Bowcock; D R Smith; L Daneshvar; P Green; L L Cavalli-Sforza; H Donis-Keller
Journal:  Nucleic Acids Res       Date:  1989-01-11       Impact factor: 16.971

2.  Physical localization of two DNA markers closely linked to the cystic fibrosis locus by pulsed-field gel electrophoresis.

Authors:  J M Rommens; S Zengerling-Lentes; B Kerem; G Melmer; M Buchwald; L C Tsui
Journal:  Am J Hum Genet       Date:  1989-12       Impact factor: 11.025

3.  Fine structure DNA mapping studies of the chromosomal region harboring the genetic defect in neurofibromatosis type I.

Authors:  P O'Connell; R J Leach; D H Ledbetter; R M Cawthon; M Culver; J R Eldridge; A K Frej; T R Holm; E Wolff; M J Thayer
Journal:  Am J Hum Genet       Date:  1989-01       Impact factor: 11.025

4.  Chromosome 7 long arm deletion breakpoints in preleukemia: mapping by pulsed field gel electrophoresis.

Authors:  J Kere
Journal:  Nucleic Acids Res       Date:  1989-02-25       Impact factor: 16.971
  4 in total

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