Shahram Rabbani1, Masoud Soleimani2, Mohammad Imani3, Mohammad Sahebjam1, Ali Ghiaseddin4, Seyed Mahdi Nassiri5, Jalil Majd Ardakani1, Maryam Tajik Rostami1, Arash Jalali1, Bahmanshir Mousanassab1, Mahsa Kheradmandi4, Seyed Hossein Ahmadi Tafti6. 1. Research Department, Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran. 2. Hematology Department, Tarbiat Modares University, Tehran, Iran. 3. Department of Novel Drug Delivery Systems, Iran Polymer and Petrochemical Institute, Tehran, Iran. 4. Chemical Engineering Department, Biomedical Engineering Division, Tarbiat Modares University, Tehran, Iran. 5. Clinical Pathology Department, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. 6. Research Department, Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: ahmadita@sina.tums.ac.ir.
Abstract
BACKGROUND: Myocardial infarction is a major problem in health system and most conventional therapy is not led to restoration of the health. Stem cell therapy is a method to regenerate the heart but today appropriate cell source and scaffold selection as extracellular matrix to achieve the best effect is disputing. AIM OF THE STUDY: In this study a combination of human Wharton jelly mesenchymal stem cells (HWJMSCs) with a novel compound consisting polyethylene glycol (PEG), hyaluronic acid and chitosan is presented to heart regeneration. METHODS: After proliferation and expansion of HWJMSCs, these cells were mixed with scaffold and injected into the infarcted rabbit myocardium. After two months cardiac function and infarcted area were evaluated. Immunohistochemistry performed for vessel count and demonstrating of differentiation ability into cardiomyocytes. To confirm this ability PCR was done. Scanning electron microscope was used to evaluate angiogenesis. RESULTS: Improving cardiac function was higher in cell/scaffold group than the others and it was confirmed by SPECT results which showed least defect size in the myocardium. There were a lot of neoangiogenesis in the target group and also cardiomyogenesis observed in cell/scaffold group. PCR results confirmed the presence of differentiated cardiomyocytes and SEM showed well developed vessel in this group. CONCLUSIONS: Comparing macroscopic and microscopic results between all groups revealed that HWJMSC in combination with this scaffold led to brilliant results regarding cardiac function, angiogenesis and cardiogenesis. It is recommended using these cells and materials for cardiac tissue engineering and regeneration therapy.
BACKGROUND:Myocardial infarction is a major problem in health system and most conventional therapy is not led to restoration of the health. Stem cell therapy is a method to regenerate the heart but today appropriate cell source and scaffold selection as extracellular matrix to achieve the best effect is disputing. AIM OF THE STUDY: In this study a combination of human Wharton jelly mesenchymal stem cells (HWJMSCs) with a novel compound consisting polyethylene glycol (PEG), hyaluronic acid and chitosan is presented to heart regeneration. METHODS: After proliferation and expansion of HWJMSCs, these cells were mixed with scaffold and injected into the infarcted rabbit myocardium. After two months cardiac function and infarcted area were evaluated. Immunohistochemistry performed for vessel count and demonstrating of differentiation ability into cardiomyocytes. To confirm this ability PCR was done. Scanning electron microscope was used to evaluate angiogenesis. RESULTS: Improving cardiac function was higher in cell/scaffold group than the others and it was confirmed by SPECT results which showed least defect size in the myocardium. There were a lot of neoangiogenesis in the target group and also cardiomyogenesis observed in cell/scaffold group. PCR results confirmed the presence of differentiated cardiomyocytes and SEM showed well developed vessel in this group. CONCLUSIONS: Comparing macroscopic and microscopic results between all groups revealed that HWJMSC in combination with this scaffold led to brilliant results regarding cardiac function, angiogenesis and cardiogenesis. It is recommended using these cells and materials for cardiac tissue engineering and regeneration therapy.
Authors: Rosa Angelica Gonzalez-Vilchis; Angelica Piedra-Ramirez; Carlos Cesar Patiño-Morales; Concepcion Sanchez-Gomez; Nohra E Beltran-Vargas Journal: Tissue Eng Regen Med Date: 2022-01-29 Impact factor: 4.169