Jian Sun1, Xiaoyue Zhang2, Renmei Li3, Zhengxi Chen2, Yuanliang Huang4, Zhenqi Chen5. 1. Resident, Department of Stomatology, Shanghai East Hospital, Tong Ji University School of Medicine, Shanghai, China, and Department of Orthodontics, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. 2. Resident, Department of Orthodontics, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. 3. Resident, Department of Stomatology, Shanghai East Hospital, Tong Ji University School of Medicine, Shanghai, China. 4. Professor, Department of Stomatology, Shanghai East Hospital, Tong Ji University School of Medicine, Shanghai, China. 5. Professor, Department of Orthodontics, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. Electronic address: orthochen@yeah.net.
Abstract
PURPOSE: Functional stimulus during orthodontic tooth movement into the grafted bone can lead to better alveolar bone grafting outcomes. The aim of this study was to analyze the biological effects of orthodontic tooth movement into the grafted alveolar cleft area with histologic staining, fluorescence staining, and real-time polymerase chain reaction (PCR). MATERIALS AND METHODS: An animal model of orthodontic tooth movement into the grafted alveolar cleft area was established in 8-week-old Sprague-Dawley rats. The animals were divided into the experimental group and the control group. Four checkpoints were observed: before orthodontic stimuli, day 1 after orthodontic stimuli, day 3 after orthodontic stimuli, and day 5 after orthodontic stimuli. The cleft bone formation conditions, including the collagen fibers and the activities of the osteoclasts and osteoblasts, were evaluated by histologic staining. The expression of tartrate-resistant acid phosphatase (TRAP), receptor activator nuclear factor κB ligand, and Runt-related transcription factor 2 was detected by real-time PCR in both groups. RESULTS: Hematoxylin-eosin staining showed that the remodeling process of iliac autografts was completed when the orthodontic stress was applied, whereas the bone tissues first showed osteoclastogenesis and then osteogenesis. On the basis of TRAP staining, the osteoclasts increased to the maximal amount on day 3 and decreased thereafter. Evidence from tetracycline fluorescence staining indicated that no obvious changes in osteoblast activity were detected at the early stage; however, it gradually increased, especially in the region close to the root surface. According to real-time PCR, the expression of TRAP increased in both the early and middle stages, that of receptor activator nuclear factor κB ligand increased in the early stage, and that of Runt-related transcription factor 2 increased in the late stage. Moreover, the results showed significant differences between the experimental and control groups. CONCLUSIONS: Orthodontic tooth movement into the alveolar cleft bone graft area promoted bone remodeling of embedded bone, thus inducing bone resorption and subsequent deposition.
PURPOSE: Functional stimulus during orthodontic tooth movement into the grafted bone can lead to better alveolar bone grafting outcomes. The aim of this study was to analyze the biological effects of orthodontic tooth movement into the grafted alveolar cleft area with histologic staining, fluorescence staining, and real-time polymerase chain reaction (PCR). MATERIALS AND METHODS: An animal model of orthodontic tooth movement into the grafted alveolar cleft area was established in 8-week-old Sprague-Dawley rats. The animals were divided into the experimental group and the control group. Four checkpoints were observed: before orthodontic stimuli, day 1 after orthodontic stimuli, day 3 after orthodontic stimuli, and day 5 after orthodontic stimuli. The cleft bone formation conditions, including the collagen fibers and the activities of the osteoclasts and osteoblasts, were evaluated by histologic staining. The expression of tartrate-resistant acid phosphatase (TRAP), receptor activator nuclear factor κB ligand, and Runt-related transcription factor 2 was detected by real-time PCR in both groups. RESULTS:Hematoxylin-eosin staining showed that the remodeling process of iliac autografts was completed when the orthodontic stress was applied, whereas the bone tissues first showed osteoclastogenesis and then osteogenesis. On the basis of TRAP staining, the osteoclasts increased to the maximal amount on day 3 and decreased thereafter. Evidence from tetracycline fluorescence staining indicated that no obvious changes in osteoblast activity were detected at the early stage; however, it gradually increased, especially in the region close to the root surface. According to real-time PCR, the expression of TRAP increased in both the early and middle stages, that of receptor activator nuclear factor κB ligand increased in the early stage, and that of Runt-related transcription factor 2 increased in the late stage. Moreover, the results showed significant differences between the experimental and control groups. CONCLUSIONS: Orthodontic tooth movement into the alveolar cleft bone graft area promoted bone remodeling of embedded bone, thus inducing bone resorption and subsequent deposition.
Authors: Stephan Christian Möhlhenrich; Kristian Kniha; Zuzanna Magnuska; Sachin Chhatwani; Benita Hermanns-Sachweh; Felix Gremse; Frank Hölzle; Gholamreza Danesh; Ali Modabber Journal: Clin Oral Investig Date: 2022-05-14 Impact factor: 3.606
Authors: Stephan Christian Möhlhenrich; Kristian Kniha; Zuzanna Magnuska; Benita Hermanns-Sachweh; Felix Gremse; Frank Hölzle; Gholamreza Danesh; Ali Modabber Journal: Sci Rep Date: 2021-06-30 Impact factor: 4.379
Authors: Stephan Christian Möhlhenrich; Kristian Kniha; Zuzanna Magnuska; Felix Gremse; Florian Peters; Gholamreza Danesh; Frank Hölzle; Ali Modabber Journal: Sci Rep Date: 2020-11-26 Impact factor: 4.379