Nastaren Abad1,2, Jens T Rosenberg2, Tangi Roussel3, Dillon C Grice1, Michael G Harrington4, Samuel C Grant1,2. 1. Department of Chemical and Biomedical Engineering, FAMU-FSU College of Engineering, Tallahassee, Florida, USA. 2. The National High Magnetic Field Laboratory, Florida State University, Tallahassee, Florida, USA. 3. Department of Chemical Physics, Weizmann Institute Science, Rehovot, Israel. 4. Molecular Neurology Program, Huntington Medical Research Institutes, Pasadena, California, USA.
Abstract
PURPOSE: This study evaluates biochemical imbalances in a rat model that reflects dysfunctional pathways in migraine. The high sensitivity and spectral dispersion available to 1 H MRS at 21.1 T expands metabolic profiling in this migraine model to include lactate (Lac), taurine (Tau), aspartate, and Gly-a mixture of glycine, glutamine, and glutamate. METHODS: Sprague-Dawley male rats were administered in situ an intraperitoneal injection of nitroglycerin (NTG) to induce the migraine analogue or saline as a control. A selective relaxation-enhanced MR spectroscopy sequence was used to target upfield metabolites from a 4-mm3 voxel for 2.5 h after injection. RESULTS: Significant increases were evident for Lac as early as 10 min after NTG injection, peaking over 50% compared with baseline and control (normalized Lac/N-acetyl aspartate with NTG = 1.54 ± 0.65 versus with saline = 0.99 ± 0.08). Tau decreased progressively in controls over 2 h after injection, but remained elevated with NTG, peaking at 105 min after injection (normalized Tau/N-acetyl aspartate with NTG = 1.10 ± 0.18 versus with saline = 0.85 ± 0.14). Total creatine under NTG showed significant decreases with time and compared with saline; Gly demonstrated temporal increases for NTG. CONCLUSIONS: These changes indicate an altered metabolic profile in the migraine analogue consistent with early changes in neural activity and/or vasodilation consistent with progressively enhanced neuroprotection and osmoregulation. Magn Reson Med 79:1266-1275, 2018.
PURPOSE: This study evaluates biochemical imbalances in a rat model that reflects dysfunctional pathways in migraine. The high sensitivity and spectral dispersion available to 1 H MRS at 21.1 T expands metabolic profiling in this migraine model to include lactate (Lac), taurine (Tau), aspartate, and Gly-a mixture of glycine, glutamine, and glutamate. METHODS: Sprague-Dawley male rats were administered in situ an intraperitoneal injection of nitroglycerin (NTG) to induce the migraine analogue or saline as a control. A selective relaxation-enhanced MR spectroscopy sequence was used to target upfield metabolites from a 4-mm3 voxel for 2.5 h after injection. RESULTS: Significant increases were evident for Lac as early as 10 min after NTG injection, peaking over 50% compared with baseline and control (normalized Lac/N-acetyl aspartate with NTG = 1.54 ± 0.65 versus with saline = 0.99 ± 0.08). Tau decreased progressively in controls over 2 h after injection, but remained elevated with NTG, peaking at 105 min after injection (normalized Tau/N-acetyl aspartate with NTG = 1.10 ± 0.18 versus with saline = 0.85 ± 0.14). Total creatine under NTG showed significant decreases with time and compared with saline; Gly demonstrated temporal increases for NTG. CONCLUSIONS: These changes indicate an altered metabolic profile in the migraine analogue consistent with early changes in neural activity and/or vasodilation consistent with progressively enhanced neuroprotection and osmoregulation. Magn Reson Med 79:1266-1275, 2018.
Authors: Roberta R Alfieri; Mara A Bonelli; Andrea Cavazzoni; Maurizio Brigotti; Claudia Fumarola; Piero Sestili; Paola Mozzoni; Giuseppe De Palma; Antonio Mutti; Domenica Carnicelli; Federica Vacondio; Claudia Silva; Angelo F Borghetti; Kenneth P Wheeler; Pier Giorgio Petronini Journal: J Physiol Date: 2006-07-27 Impact factor: 5.182
Authors: Xianghong Arakaki; Hailey Foster; Lei Su; Huy Do; Andrew J Wain; Alfred N Fonteh; Feimeng Zhou; Michael G Harrington Journal: Brain Res Date: 2011-05-23 Impact factor: 3.252
Authors: G D'Andrea; A R Cananzi; R Joseph; M Morra; F Zamberlan; F Ferro Milone; S Grunfeld; K M Welch Journal: Cephalalgia Date: 1991-09 Impact factor: 6.292
Authors: Samaira Younis; Anders Hougaard; Mark B Vestergaard; Henrik B W Larsson; Messoud Ashina Journal: Curr Opin Neurol Date: 2017-06 Impact factor: 5.710
Authors: D Grimaldi; C Tonon; S Cevoli; G Pierangeli; E Malucelli; G Rizzo; S Soriani; P Montagna; B Barbiroli; R Lodi; P Cortelli Journal: Cephalalgia Date: 2010-02-01 Impact factor: 6.292
Authors: L Becerra; R Veggeberg; A Prescot; J E Jensen; P Renshaw; S Scrivani; E L H Spierings; R Burstein; D Borsook Journal: Neuroimage Clin Date: 2016-03-31 Impact factor: 4.881