Tatsuya Ohkawara1, Hiroshi Takeda2, Jun Nishihira3. 1. Pathophysiology and Therapeutics, Hokkaido University, Faculty of Pharmaceutical Sciences, Kita-12, Nishi-6, Kita-ku, Sapporo 060-8638, Japan. Electronic address: tokawara@pharm.hokudai.ac.jp. 2. Pathophysiology and Therapeutics, Hokkaido University, Faculty of Pharmaceutical Sciences, Kita-12, Nishi-6, Kita-ku, Sapporo 060-8638, Japan. 3. Department of Medical Management and Informatics, Faculty of Business Administration and Information Science, Hokkaido Information University, Nishinopporo, 59-2, Ebetsu 069-8585, Japan.
Abstract
AIMS: Pancreatitis is characterized by inflammatory disease with severe tissue injury in pancreas, and the incidence of pancreatitis has been recently increasing. Although several treatments of acute pancreatitis have been developed, some patients have been resistant to current therapy. Chlorogenic acid (CGA) is one of the polyphenols, and is known to have an anti-inflammatory effect. In this study, we investigated the effects of CGA on experimental pancreatitis in mice. MATERIALS AND METHODS: Pancreatitis was induced by twice injection of l-arginine (5g/kg body weight). Mice were intraperitoneally injected with CGA (20mg/kg or 40mg/kg) 1h before administration of l-arginine. KEY FINDINGS: Administration of 40mg/kg of CGA decreased the histological severity of pancreatitis and pancreatitis-associated lung injury. Moreover, administration of CGA inhibited the levels of pancreatic enzyme activity. Interestingly, CGA reduced the serum and pancreatic levels of macrophage migration inhibitory factor (MIF) in mice with l-arginine-induced pancreatitis. SIGNIFICANCE: Our results suggest that CGA has an anti-inflammatory effect on l-arginine-induced pancreatitis and pancreatitis-associated lung injury.
AIMS: Pancreatitis is characterized by inflammatory disease with severe tissue injury in pancreas, and the incidence of pancreatitis has been recently increasing. Although several treatments of acute pancreatitis have been developed, some patients have been resistant to current therapy. Chlorogenic acid (CGA) is one of the polyphenols, and is known to have an anti-inflammatory effect. In this study, we investigated the effects of CGA on experimental pancreatitis in mice. MATERIALS AND METHODS:Pancreatitis was induced by twice injection of l-arginine (5g/kg body weight). Mice were intraperitoneally injected with CGA (20mg/kg or 40mg/kg) 1h before administration of l-arginine. KEY FINDINGS: Administration of 40mg/kg of CGA decreased the histological severity of pancreatitis and pancreatitis-associated lung injury. Moreover, administration of CGA inhibited the levels of pancreatic enzyme activity. Interestingly, CGA reduced the serum and pancreatic levels of macrophage migration inhibitory factor (MIF) in mice with l-arginine-induced pancreatitis. SIGNIFICANCE: Our results suggest that CGA has an anti-inflammatory effect on l-arginine-induced pancreatitis and pancreatitis-associated lung injury.