Activity of Bacillus thuringiensis Cry1Ie2, Cry2Ac7, Vip3Aa11 and Cry7Ab3 proteins against Anticarsia gemmatalis, Chrysodeixis includens and Ceratoma trifurcata.

Transgenic soybean producing the Cry1Ac insecticidal protein from the bacterium Bacillus thuringiensis is used to control larvae of the velvetbean caterpillar (Anticarsia gemmatalis Hübner) and the soybean looper [Chrysodeixis includens (Walker)]. The main threat to the sustainability of this technology is the development of resistance, which could be delayed by using pyramiding of diverse Bt insecticidal genes. We report high activity of Cry2Ac7 and Vip3Aa11 but not Cry1Ie2 against larvae of A. gemmatalis and C. includens. In addition, we also report anti-feeding activity of Cry1Ie2 and Cry7Ab3 in adults of the bean leaf beetle [Ceratoma trifurcata (Foster)], an alternative pest of soybean.