Literature DB >> 28918068

A simple and rapid DNA extraction method for Chlamydia trachomatis detection from urogenital swabs.

Matthew A Butzler1, Jennifer L Reed1, Sally M McFall2.   

Abstract

A highly sensitive and specific Chlamydia trachomatis (CT) diagnostic test was developed by combining filtration isolation of nucleic acid (FINA) extraction with quantitative polymerase chain reaction including an internal control to identify test inhibition. A pilot study of 40 clinical specimens yielded 100% sensitivity and specificity.
Copyright © 2017 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  Chlamydia trachomatis; Diagnosis; Quantitative PCR; Sample prep; Sexually transmitted disease

Mesh：

Substances：

Year:  2017        PMID： 28918068      PMCID： PMC5679254          DOI： 10.1016/j.diagmicrobio.2017.08.007

Source DB:  PubMed          Journal:  Diagn Microbiol Infect Dis        ISSN： 0732-8893            Impact factor:   2.803


  12 in total

Review 1.  Performing quantitative reverse-transcribed polymerase chain reaction experiments.

Authors:  Georges Lutfalla; Gilles Uze
Journal:  Methods Enzymol       Date:  2006       Impact factor: 1.600

2.  A RealTime HIV-1 viral load assay for automated quantitation of HIV-1 RNA in genetically diverse group M subtypes A-H, group O and group N samples.

Authors:  Ning Tang; Shihai Huang; John Salituro; Wai-Bing Mak; Gavin Cloherty; Julie Johanson; Yu Hong Li; George Schneider; John Robinson; John Hackett; Priscilla Swanson; Klara Abravaya
Journal:  J Virol Methods       Date:  2007-08-17       Impact factor: 2.014

3.  Primer sequence disclosure: a clarification of the MIQE guidelines.

Authors:  Stephen A Bustin; Vladimir Benes; Jeremy A Garson; Jan Hellemans; Jim Huggett; Mikael Kubista; Reinhold Mueller; Tania Nolan; Michael W Pfaffl; Gregory L Shipley; Jo Vandesompele; Carl T Wittwer
Journal:  Clin Chem       Date:  2011-03-18       Impact factor: 8.327

4.  A point-of-care PCR test for HIV-1 detection in resource-limited settings.

Authors:  Sujit R Jangam; Abhishek K Agarwal; Kunal Sur; David M Kelso
Journal:  Biosens Bioelectron       Date:  2012-10-17       Impact factor: 10.618

5.  A simple and rapid DNA extraction method from whole blood for highly sensitive detection and quantitation of HIV-1 proviral DNA by real-time PCR.

Authors:  Sally M McFall; Robin L Wagner; Sujit R Jangam; Douglas H Yamada; Diana Hardie; David M Kelso
Journal:  J Virol Methods       Date:  2015-02-12       Impact factor: 2.014

6.  A common plasmid of Chlamydia trachomatis.

Authors:  L Palmer; S Falkow
Journal:  Plasmid       Date:  1986-07       Impact factor: 3.466

Review 7.  The Swedish new variant of Chlamydia trachomatis.

Authors:  Magnus Unemo; Ian N Clarke
Journal:  Curr Opin Infect Dis       Date:  2011-02       Impact factor: 4.915

8.  Sexually transmitted infections in women.

Authors:  Barbara Van Der Pol
Journal:  Scand J Clin Lab Invest Suppl       Date:  2014

9.  Rapid, point-of-care extraction of human immunodeficiency virus type 1 proviral DNA from whole blood for detection by real-time PCR.

Authors:  Sujit R Jangam; Douglas H Yamada; Sally M McFall; David M Kelso
Journal:  J Clin Microbiol       Date:  2009-08       Impact factor: 5.948

10.  What qualities are most important to making a point of care test desirable for clinicians and others offering sexually transmitted infection testing?

Authors:  Yu-Hsiang Hsieh; Charlotte A Gaydos; M Terry Hogan; O Manuel Uy; Joany Jackman; Mary Jett-Goheen; Ariel Albertie; Derek T Dangerfield; Celia R Neustadt; Zachary S Wiener; Anne M Rompalo
Journal:  PLoS One       Date:  2011-04-29       Impact factor: 3.240
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