Analysis of glutathione in the presence of acetaminophen and tyrosine via an amplified electrode with MgO/SWCNTs as a sensor in the hemolyzed erythrocyte.

In this study, we investigated the effect of adding MgO/SWCNTs and 2-Chloro-N'-[1-(2,5-dihydroxyphenyl) methylidene]aniline (2-CDHPMA) to a carbon paste matrix, which could act as a voltammetric sensor for the analysis of glutathione. The electrocatalytic interaction between 2-CDHPMA and glutathione was used as a factor for the analysis of this biological compound in real samples. In addition, the presence of MgO/SWCNTs can help in increasing the sensitivity of the fabricated sensor and for obtaining a low limit of detection in the analysis of glutathione. When the carbon-paste electrode was amplified with MgO/SWCNTs and 2-CDHPMA (CPE/MgO/SWCNTs/2-CDHPMA), it showed a good selectivity for the analysis of glutathione in the presence of acetaminophen and tyrosine with the separated peak potentials at ~ 280mV, ~ 570mV, and ~ 880mV for the first time. We detected a linear dynamic range between 0.05 and 700.0μmolL-1 with the limit of detection set at ~ 10 ± 0.3nmolL-1 for the analysis of glutathione by the square-wave voltammetric method. The CPE/MgO/SWCNTs/2-CDHPMA showed a high quality of detection of glutathione in the hemolyzed erythrocyte sample.