Mingming Yu1, Inas A Abdallah1,2, Soo Hyeon Shin1, Dana C Hammell1, Audra L Stinchcomb1, Hazem E Hassan1,3. 1. Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland, Baltimore, MD, USA. 2. Department of Analytical Chemistry, Faculty of Pharmacy, University of Sadat City, Sadat City, Egypt. 3. Department of Pharmaceutics & Industrial Pharmacy, Faculty of Pharmacy, Helwan University, Cairo, Egypt.
Abstract
AIM: Fentanyl is an opioid agonist used for acute and chronic pain management. In this report, a highly sensitive and simple LC-MS/MS method using Hydrophilic Interaction Chromatography (HILIC) column was validated and used for fentanyl quantification in human serum. RESULTS: The isocratic mobile phase was composed of acetonitrile: 10 mM ammonium formate buffer (pH = 3.2; 90:10, v/v). The assay was linear over a concentration range of 10-10,000 pg/ml. The accuracy of the validation method ranged from 93.2 to 107%, and the precision was within 6.4%. Fentanyl was stable during short- and long-term storage. CONCLUSION: The assay has been successfully applied to serum samples obtained from healthy subjects of a fentanyl transdermal pharmacokinetic study.
AIM: Fentanyl is an opioid agonist used for acute and chronic pain management. In this report, a highly sensitive and simple LC-MS/MS method using Hydrophilic Interaction Chromatography (HILIC) column was validated and used for fentanyl quantification in human serum. RESULTS: The isocratic mobile phase was composed of acetonitrile: 10 mM ammonium formate buffer (pH = 3.2; 90:10, v/v). The assay was linear over a concentration range of 10-10,000 pg/ml. The accuracy of the validation method ranged from 93.2 to 107%, and the precision was within 6.4%. Fentanyl was stable during short- and long-term storage. CONCLUSION: The assay has been successfully applied to serum samples obtained from healthy subjects of a fentanyl transdermal pharmacokinetic study.
Authors: Anil R Maharaj; Huali Wu; Kanecia O Zimmerman; David G Speicher; Janice E Sullivan; Kevin Watt; Amira Al-Uzri; Elizabeth H Payne; Jinson Erinjeri; Susan Lin; Barrie Harper; Chiara Melloni; Christoph P Hornik Journal: J Clin Pharmacol Date: 2019-12-08 Impact factor: 2.860