| Literature DB >> 28913799 |
Caroline Tassy1, Pierre Barret2.
Abstract
The wheat genome encodes some 100,000 genes. To understand how the expression of these genes is regulated it will be necessary to carry out many genetic transformation experiments. Robust protocols that allow scientists to transform a wide range of wheat genotypes are therefore required. In this chapter, we describe a protocol for biolistic transformation of wheat that uses immature embryos and small quantities of DNA cassettes. An original method for DNA cassette purification is also described. This protocol can be used to transform a wide range of wheat genotypes and other related species.Entities:
Keywords: Biolistic transformation; Gene cassette; Wheat
Mesh：
Year: 2017 PMID： 28913799 DOI： 10.1007/978-1-4939-7337-8_9
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745