Yujuan Han1, Peixin Gao1, Wengong Yu1, Xinzhi Lu2. 1. Key Laboratory of Glycoscience & Glycotechnology of Shandong Province; Key Laboratory of Marine Drugs, Chinese Ministry of Education; Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology; Department of Glycobiology; School of Medicine and Pharmacy, Ocean University of China, 5 Yushan Road, Qingdao, 266003, China. 2. Key Laboratory of Glycoscience & Glycotechnology of Shandong Province; Key Laboratory of Marine Drugs, Chinese Ministry of Education; Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology; Department of Glycobiology; School of Medicine and Pharmacy, Ocean University of China, 5 Yushan Road, Qingdao, 266003, China. luxinzhi@ouc.edu.cn.
Abstract
OBJECTIVE: To determine the effects of the extra N-terminal seven-amino-acid sequence on the function of chitosanase CsnA. RESULTS: Sequence and structure analysis indicated that the mature CsnA contains a seven-amino-acid extension in a disordered form at the N-terminus. To determine the function of this sequence, both mature CsnA and its N-terminus-truncated mutant, CsnAΔN, were expressed in Escherichia coli and characterized. Compared with CsnAΔN, CsnA exhibited a 15 °C higher temperature optimum, enhanced pH stability, thermostability and catalytic efficiency. The underlying mechanisms responsible for these changes were analyzed by circular dichroism (CD) spectroscopy. CD analysis revealed that the deletion of the N-terminal sequence resulted in a decrease in the Tm of 4.3 °C and this sequence altered the secondary structure of the enzyme. CONCLUSIONS: The N-terminal sequence is essential for the stability and activity of chitosanase CsnA.
OBJECTIVE: To determine the effects of the extra N-terminal seven-amino-acid sequence on the function of chitosanase CsnA. RESULTS: Sequence and structure analysis indicated that the mature CsnA contains a seven-amino-acid extension in a disordered form at the N-terminus. To determine the function of this sequence, both mature CsnA and its N-terminus-truncated mutant, CsnAΔN, were expressed in Escherichia coli and characterized. Compared with CsnAΔN, CsnA exhibited a 15 °C higher temperature optimum, enhanced pH stability, thermostability and catalytic efficiency. The underlying mechanisms responsible for these changes were analyzed by circular dichroism (CD) spectroscopy. CD analysis revealed that the deletion of the N-terminal sequence resulted in a decrease in the Tm of 4.3 °C and this sequence altered the secondary structure of the enzyme. CONCLUSIONS: The N-terminal sequence is essential for the stability and activity of chitosanase CsnA.