Interaction between enkephalin and dopamine in the avian retina.

Biochemical and pharmacological techniques were utilized to investigate the interaction between the enkephalinergic and dopaminergic systems in the chicken retina. Exogenously applied enkephalin and its analogues were observed to inhibit the release of preloaded dopamine from the retina. This inhibition was concentration-dependent and was suppressed by the opiate antagonist, naloxone. The relationship between enkephalinergic and dopaminergic amacrine cells was studied in retinas which were subjected to 6-hydroxydopamine (6-OHDA) treatments. 6-OHDA degenerated approximately 80-90% of those cells which exhibit high affinity uptake of [3H]dopamine. In 6-OHDA-treated retinas, the capacity of 3H-labelled [D-Ala2]methionine enkephalinamide to bind specifically to opiate receptors was substantially reduced (only 70-75% of the control). Scatchard analyses and ligand displacement studies indicated that this decrease in binding was due to a reduction in the number of opiate receptors. Taken together, these observations strongly indicate that a fraction of the opiate receptors in the chicken retina (25-30%) are closely associated with the population of dopaminergic amacrine cells.