Nurfadly Zain1, Suhartono Taat Putra2, Umar Zein3, Herman Hariman4. 1. Department of Tropical Medicine, Faculty of Medicine, Muhammadiyah University of North Sumatera, Medan, Indonesia. 2. Department of Pathobiology, Faculty of Medicine, Airlangga University, Surabaya, Indonesia. 3. Department of Internal Medicine, Faculty of Medicine, Islamic University of North Sumatera Medan, Medan, Indonesia. 4. Department of Clinical Pathology, Faculty of Medicine, University of North Sumatera, Medan, Indonesia.
Abstract
BACKGROUND: The apoptosis of microvascular endothelial cells causes plasma leakage in dengue haemorrhagic fever patients. The soluble Fas ligand is a protein with molecular weight of 40 kDa that acts as a mediator of apoptosis. This study aimed to prove whether soluble Fas ligand can be used as a potential marker to predict the severity of dengue infection by comparing the soluble Fas ligand levels in dengue fever (DF) and dengue haemorrhagic fever (DHF) patients early in the course of illness. METHOD: This was a prospective study. It included 42 dengue patients (22 DF patients and 20 DHF patients) and 20 healthy people as a control group. The soluble Fas ligand was measured by the enzyme-linked immunosorbent assay (ELISA). RESULT: Soluble Fas ligand was increased significantly (P < 0.001) in DHF patients (median = 130.19, IQR = 36.26) compared to DF patients (median = 104.73, IQR = 53.94) and the control group (median = 87.16, IQR = 24.91). CONCLUSION: Soluble Fas ligand can be used as a potential marker to predict the severity of dengue infection in the early course of the illness. However, a larger sample size and further objective studies are needed to confirm these findings.
BACKGROUND: The apoptosis of microvascular endothelial cells causes plasma leakage in dengue haemorrhagic feverpatients. The soluble Fas ligand is a protein with molecular weight of 40 kDa that acts as a mediator of apoptosis. This study aimed to prove whether soluble Fas ligand can be used as a potential marker to predict the severity of dengue infection by comparing the soluble Fas ligand levels in dengue fever (DF) and dengue haemorrhagic fever (DHF) patients early in the course of illness. METHOD: This was a prospective study. It included 42 dengue patients (22 DF patients and 20 DHF patients) and 20 healthy people as a control group. The soluble Fas ligand was measured by the enzyme-linked immunosorbent assay (ELISA). RESULT: Soluble Fas ligand was increased significantly (P < 0.001) in DHF patients (median = 130.19, IQR = 36.26) compared to DF patients (median = 104.73, IQR = 53.94) and the control group (median = 87.16, IQR = 24.91). CONCLUSION: Soluble Fas ligand can be used as a potential marker to predict the severity of dengue infection in the early course of the illness. However, a larger sample size and further objective studies are needed to confirm these findings.
Authors: Michael G Brown; Yan Y Huang; Jean S Marshall; Christine A King; David W Hoskin; Robert Anderson Journal: J Leukoc Biol Date: 2008-09-22 Impact factor: 4.962
Authors: Judit Kocsis; Tamás Mészáros; Balázs Madaras; Eva Katalin Tóth; Szilárd Kamondi; Péter Gál; Lilian Varga; Zoltán Prohászka; George Füst Journal: Cell Stress Chaperones Date: 2010-08-22 Impact factor: 3.667