| Literature DB >> 28893500 |
Haibo Yuan1, Jianghua Li1, Hyun-Dong Shin2, Guocheng Du3, Jian Chen3, Zhongping Shi4, Long Liu1.
Abstract
2,5-Furandicarboxylic acid (FDCA) is a promising bio-based building block and can be produced by biotransformation of 5-hydroxymethylfurfural (HMF). To improve the FDCA production, two genes-one encoding HMF oxidase (HMFO; from Methylovorus sp. strain MP688) and another encoding for HMF/Furfural oxidoreductase (HmfH; from Cupriavidus basilensis HMF14)-were introduced into Raoultella ornithinolytica BF60. The FDCA production in the engineered whole-cell biocatalyst increased from 51.0 to 93.6mM, and the molar conversion ratio of HMF to FDCA increased from 51.0 to 93.6%.Entities:
Keywords: 2,5-Furandicarboxylic acid; 5-Hydroxymethylfurfural oxidase; Biotransformation; HMF/furfural oxidoreductase; Raoultella ornithinolytica BF60
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Year: 2017 PMID: 28893500 DOI: 10.1016/j.biortech.2017.08.166
Source DB: PubMed Journal: Bioresour Technol ISSN: 0960-8524 Impact factor: 9.642