Michele Tatiana Pereira Tomitão1,2, Sergio Carlos Nahas1,3, Marcia Saldanha Kubrusly4, Tatiane Katsue Furuya2,5, Marcio Augusto Diniz6, Suely Kazue Nagahashi Marie7, Adriana Vaz Safatle-Ribeiro1,3, José Eluf-Neto8, Ivan Cecconello1, Ulysses Ribeiro Junior1,3. 1. Departamento de Gastroenterologia, Faculdade de Medicina da Universidade de São Paulo, São Paulo/SP, Brasil. 2. Centro de Investigação Translacional em Oncologia, Instituto do Câncer do Estado de São Paulo, São Paulo/SP, Brasil. 3. Instituto do Câncer do Estado de São Paulo, São Paulo/SP, Brasil. 4. Divisão de Transplante de Órgãos Digestivos LIM 37, Faculdade de Medicina da Universidade de São Paulo, São Paulo/SP, Brasil. 5. Departamento de Radiologia e Oncologia LIM 24, Faculdade de Medicina da Universidade de São Paulo, São Paulo/SP, Brasil. 6. Cedars-Sinai Samuel Oschin Comprehensive Cancer Institute, Los Angeles, CA, USA. 7. Departamento de Neurologia LIM 15, Faculdade de Medicina da Universidade de São Paulo, São Paulo/SP, Brasil. 8. Departamento de Medicina Preventiva, Faculdade de Medicina da Universidade de São Paulo, São Paulo/SP, Brasil.
Abstract
BACKGROUND: Multi-ethnicity of Brazilian population displays high levels of genomic diversity. Polymorphism may detect people at higher risk of developing cancer, distinctive response to treatment, and prognosis. Cyclooxygenase-2 (COX-2) is induced in response to growth factors and cytokines, and is expressed in inflammatory diseases, precancerous lesions and colorectal cancer (CRC). The aim of this study was to evaluate the influence of COX-2 -1195A > G and 8473T > C polymorphisms as a risk factor of developing CRC. METHODS: We evaluated COX-2 Single Nucleotide Polymorphism (SNP) of 230 CRC patients and 196 healthy controls by Real-Time Polymerase Chain Reaction. RESULTS: Populations were in Hardy-Weinberg equilibrium (HWE), except for control group of 8473T > C SNP. The frequencies were similar in both groups for genotypes and haplotypes. There was no association between studied polymorphisms and risk of CRC. CONCLUSIONS: The gene polymorphisms studied do not participate in the genetic susceptibility to CRC in a Brazilian population.
BACKGROUND: Multi-ethnicity of Brazilian population displays high levels of genomic diversity. Polymorphism may detect people at higher risk of developing cancer, distinctive response to treatment, and prognosis. Cyclooxygenase-2 (COX-2) is induced in response to growth factors and cytokines, and is expressed in inflammatory diseases, precancerous lesions and colorectal cancer (CRC). The aim of this study was to evaluate the influence of COX-2 -1195A > G and 8473T > C polymorphisms as a risk factor of developing CRC. METHODS: We evaluated COX-2 Single Nucleotide Polymorphism (SNP) of 230 CRC patients and 196 healthy controls by Real-Time Polymerase Chain Reaction. RESULTS: Populations were in Hardy-Weinberg equilibrium (HWE), except for control group of 8473T > C SNP. The frequencies were similar in both groups for genotypes and haplotypes. There was no association between studied polymorphisms and risk of CRC. CONCLUSIONS: The gene polymorphisms studied do not participate in the genetic susceptibility to CRC in a Brazilian population.
Entities:
Keywords:
Polymorphism; colorectal cancer (CRC); cyclooxygenase-2 (COX-2); genes; risk; single nucleotide polymorphism (SNP); susceptibility
Authors: Juliët H Hoff; Rene H M te Morsche; Hennie M J Roelofs; Elise M J van der Logt; Fokko M Nagengast; Wilbert H M Peters Journal: World J Gastroenterol Date: 2009-09-28 Impact factor: 5.742