Literature DB >> 28889396

Visualization and Quantitative Measurement of Drug-Induced Platinum Adducts in the Nuclear DNA of Individual Cells by an Immuno-Cytological Assay.

Margarita Melnikova1, Jürgen Thomale2.   

Abstract

Immunocytological staining with adduct-specific antibodies allows the visualization and measurement of structurally defined types of DNA damage in the nuclei of individual cells. Here we describe an immunocytological assay (ICA) procedure for the localization and quantification of such damage, in particular induced by platinum-based anticancer drugs, in cell lines , in primary cell suspensions and in frozen tissue sections.

Keywords:  Cisplatin; DNA adducts; Immunofluorescence; Monoclonal antibo d y; Quantitative image analysis; Single cell analysis

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Year:  2018        PMID： 28889396     DOI： 10.1007/978-1-4939-7234-0_24

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  2 in total

1.  Diphenhydramine increases the therapeutic window for platinum drugs by simultaneously sensitizing tumor cells and protecting normal cells.

Authors:  Margarita Melnikova; Ulrike Sophie Wauer; Diana Mendus; Ralf Axel Hilger; Trudy G Oliver; Kim Mercer; Björn Oliver Gohlke; Kati Erdmann; Dieter Niederacher; Hans Neubauer; Paul Buderath; Pauline Wimberger; Jan Dominik Kuhlmann; Jürgen Thomale
Journal:  Mol Oncol       Date:  2020-03-10       Impact factor: 6.603

2.  HSP90 Inhibition Synergizes with Cisplatin to Eliminate Basal-like Pancreatic Ductal Adenocarcinoma Cells.

Authors:  Katharina M Ewers; Shilpa Patil; Waltraut Kopp; Jürgen Thomale; Tabea Quilitz; Anna Magerhans; Xin Wang; Elisabeth Hessmann; Matthias Dobbelstein
Journal:  Cancers (Basel)       Date:  2021-12-07       Impact factor: 6.639
  2 in total

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