Literature DB >> 28889284

High-Performance Liquid Chromatography (HPLC)-Based Detection and Quantitation of Cellular c-di-GMP.

Olga E Petrova1, Karin Sauer2.   

Abstract

The modulation of c-di-GMP levels plays a vital role in the regulation of various processes in a wide array of bacterial species. Thus, investigation of c-di-GMP regulation requires reliable methods for the assessment of c-di-GMP levels and turnover. Reversed-phase high-performance liquid chromatography (RP-HPLC) analysis has become a commonly used approach to accomplish these goals. The following describes the extraction and HPLC-based detection and quantification of c-di-GMP from Pseudomonas aeruginosa samples, a procedure that is amenable to modifications for the analysis of c-di-GMP in other bacterial species.

Entities:  

Keywords:  Biofilm; Cyclic dinucleotides; High-performance liquid chromatography; Ion exchange; Reversed phase; bis-(3′,5′)-cyclic dimeric guanosine monophosphate; c-di-GMP

Mesh：

Substances：

Year:  2017        PMID： 28889284      PMCID： PMC5702474          DOI： 10.1007/978-1-4939-7240-1_4

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  22 in total

1.  Acetylornithinase of Escherichia coli: partial purification and some properties.

Authors:  H J VOGEL; D M BONNER
Journal:  J Biol Chem       Date:  1956-01       Impact factor: 5.157

Review 2.  Prevailing concepts of c-di-GMP signaling.

Authors:  Ute Römling; Roger Simm
Journal:  Contrib Microbiol       Date:  2009-06-02

3.  Identification and characterization of a phosphodiesterase that inversely regulates motility and biofilm formation in Vibrio cholerae.

Authors:  Xianxian Liu; Sinem Beyhan; Bentley Lim; Roger G Linington; Fitnat H Yildiz
Journal:  J Bacteriol       Date:  2010-07-09       Impact factor: 3.490

4.  The ubiquitous protein domain EAL is a cyclic diguanylate-specific phosphodiesterase: enzymatically active and inactive EAL domains.

Authors:  Andrew J Schmidt; Dmitri A Ryjenkov; Mark Gomelsky
Journal:  J Bacteriol       Date:  2005-07       Impact factor: 3.490

5.  The cyclic diguanylic acid regulatory system of cellulose synthesis in Acetobacter xylinum. Chemical synthesis and biological activity of cyclic nucleotide dimer, trimer, and phosphothioate derivatives.

Authors:  P Ross; R Mayer; H Weinhouse; D Amikam; Y Huggirat; M Benziman; E de Vroom; A Fidder; P de Paus; L A Sliedregt
Journal:  J Biol Chem       Date:  1990-11-05       Impact factor: 5.157

6.  Quantification of cyclic dinucleotides by reversed-phase LC-MS/MS.

Authors:  Heike Burhenne; Volkhard Kaever
Journal:  Methods Mol Biol       Date:  2013

7.  Quantitative determination of cyclic diguanosine monophosphate concentrations in nucleotide extracts of bacteria by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry.

Authors:  Roger Simm; Michael Morr; Uwe Remminghorst; Mats Andersson; Ute Römling
Journal:  Anal Biochem       Date:  2008-12-24       Impact factor: 3.365

8.  PAS domain residues and prosthetic group involved in BdlA-dependent dispersion response by Pseudomonas aeruginosa biofilms.

Authors:  Olga E Petrova; Karin Sauer
Journal:  J Bacteriol       Date:  2012-08-24       Impact factor: 3.490

9.  NO-induced biofilm dispersion in Pseudomonas aeruginosa is mediated by an MHYT domain-coupled phosphodiesterase.

Authors:  Yi Li; Sabrina Heine; Michael Entian; Karin Sauer; Nicole Frankenberg-Dinkel
Journal:  J Bacteriol       Date:  2013-05-31       Impact factor: 3.490

10.  A simple fluorometric method for cAMP: application to studies of brain adenylate cyclase activity.

Authors:  W Wojcik; M Olianas; M Parenti; S Gentleman; N H Neff
Journal:  J Cyclic Nucleotide Res       Date:  1981
View more
  6 in total

1.  Regulation of flagellar motor switching by c-di-GMP phosphodiesterases in Pseudomonas aeruginosa.

Authors:  Lingyi Xin; Yukai Zeng; Shuo Sheng; Rachel Andrea Chea; Qiong Liu; Hoi Yeung Li; Liang Yang; Linghui Xu; Keng-Hwee Chiam; Zhao-Xun Liang
Journal:  J Biol Chem       Date:  2019-07-26       Impact factor: 5.157

Review 2.  Characterization and Analysis of Food-Sourced Carbohydrates.

Authors:  Leonie J Kiely; Rita M Hickey
Journal:  Methods Mol Biol       Date:  2022

3.  Development of Ratiometric Bioluminescent Sensors for in Vivo Detection of Bacterial Signaling.

Authors:  Andrew B Dippel; Wyatt A Anderson; Jin Hwan Park; Fitnat H Yildiz; Ming C Hammond
Journal:  ACS Chem Biol       Date:  2020-03-25       Impact factor: 5.100

4.  Acute toxicity analysis of an inhibitor of BCL2, Disarib, in rats.

Authors:  Shivangi Sharma; Kontham Kulangara Varsha; Ujjayinee Ray; Humaira Siddiqua; Anjana Elizabeth Jose; Sridhar Muninarasimaiah; Sathees C Raghavan; Bibha Choudhary
Journal:  Sci Rep       Date:  2021-05-11       Impact factor: 4.379

5.  RstA, a two-component response regulator, plays important roles in multiple virulence-associated processes in enterohemorrhagic Escherichia coli O157:H7.

Authors:  Yutao Liu; Shujie Li; Wendi Li; Peisheng Wang; Peng Ding; Lingyu Li; Junyue Wang; Pan Yang; Qian Wang; Tingting Xu; Yingying Xiong; Bin Yang
Journal:  Gut Pathog       Date:  2019-11-01       Impact factor: 4.181

Review 6.  The Processing, Gene Regulation, Biological Functions, and Clinical Relevance of N4-Acetylcytidine on RNA: A Systematic Review.

Authors:  Gehui Jin; Mingqing Xu; Mengsha Zou; Shiwei Duan
Journal:  Mol Ther Nucleic Acids       Date:  2020-02-08       Impact factor: 8.886
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.