Aldl de Andrade1,2, C E de Oliveira2, M R Dourado2, Ccs Macedo2, F V Winck3, A F Paes Leme3, T Salo2,4,5, R D Coletta2, R de Almeida Freitas1, H C Galvão1. 1. Department of Dentistry, Federal University of Rio Grande do Norte, Natal, Brazil. 2. Department of Oral Diagnosis, School of Dentistry, University of Campinas, Piracicaba, Brazil. 3. Mass Spectrometry Laboratory, Biosciences National Laboratory, LNBio, CNPEM, Campinas, Brazil. 4. Unit of Cancer Research and Translational Medicine, Faculty of Medicine, Medical Research Center Oulu, Oulu University Hospital, University of Oulu, Oulu, Finland. 5. Department of Pathology, Institute of Oral and Maxillofacial Disease, HUSLAB, Helsinki University Hospital, University of Helsinki, Helsinki, Finland.
Abstract
BACKGROUND: A new intercellular communication mode established by neoplastic cells and tumor microenvironment components is based on extracellular vesicles (EVs). However, the biological effects of the EVs released by tumor cells on angiogenesis are not completely understood. Here, we aimed to understand the biological effects of EVs isolated from two cell lines of oral squamous cell carcinoma (OSCC) (SCC15 and HSC3) on endothelial cell tubulogenesis. METHODS: OSCC-derived EVs were isolated with a polymer-based precipitation method, quantified using nanoparticle tracking analysis and verified for EV markers by dot blot. Functional assays were performed to assess the angiogenic potential of the OSCC-derived EVs. RESULTS: The results showed that EVs derived from both cell lines displayed typical spherical-shaped morphology and expressed the EV markers CD63 and Annexin II. Although the average particle concentration and size were quite similar, SCC15-derived EVs promoted a pronounced tubular formation associated with significant migration and apoptosis rates of the endothelial cells, whereas EVs derived from HSC3 cells inhibited significantly endothelial cell tubulogenesis and proliferation. CONCLUSION: The findings of this study reveal that EVs derived from different OSCC cell lines by a polymer-based precipitation method promote pro- or anti-angiogenic effects.
BACKGROUND: A new intercellular communication mode established by neoplastic cells and tumor microenvironment components is based on extracellular vesicles (EVs). However, the biological effects of the EVs released by tumor cells on angiogenesis are not completely understood. Here, we aimed to understand the biological effects of EVs isolated from two cell lines of oral squamous cell carcinoma (OSCC) (SCC15 and HSC3) on endothelial cell tubulogenesis. METHODS: OSCC-derived EVs were isolated with a polymer-based precipitation method, quantified using nanoparticle tracking analysis and verified for EV markers by dot blot. Functional assays were performed to assess the angiogenic potential of the OSCC-derived EVs. RESULTS: The results showed that EVs derived from both cell lines displayed typical spherical-shaped morphology and expressed the EV markers CD63 and Annexin II. Although the average particle concentration and size were quite similar, SCC15-derived EVs promoted a pronounced tubular formation associated with significant migration and apoptosis rates of the endothelial cells, whereas EVs derived from HSC3 cells inhibited significantly endothelial cell tubulogenesis and proliferation. CONCLUSION: The findings of this study reveal that EVs derived from different OSCC cell lines by a polymer-based precipitation method promote pro- or anti-angiogenic effects.
Authors: Cristina P R Xavier; Hugo R Caires; Mélanie A G Barbosa; Rui Bergantim; José E Guimarães; M Helena Vasconcelos Journal: Cells Date: 2020-05-06 Impact factor: 6.600