Min Jae Kim1, Yoo Yeon Kim2, Janet Ren Chao3, Hae Sang Park1,4, Jiwon Chang1,4, Dawoon Oh5, Jae Jun Lee4,6, Tae Chun Kang7, Jun-Gyo Suh8, Jun Ho Lee9,4. 1. Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Hallym University, Chuncheon, Republic of Korea. 2. Department of Medical Genetics, College of Medicine, Hallym University, Chuncheon, Republic of Korea. 3. School of Medicine, George Washington University, Washington, DC, U.S.A. 4. Institute of New Frontier Research, Hallym University College of Medicine, Chuncheon, Republic of Korea. 5. Department of Anesthesiology and Pain Medicine, Dongtan Sacred Heart Hospital, Hallym University, Dongtan, Republic of Korea. 6. Department of Anesthesiology and Pain Medicine, College of Medicine, Hallym University, Chuncheon, Republic of Korea. 7. Department of Anatomy and Neurobiology, College of Medicine, Hallym University, Chuncheon, Republic of Korea. 8. Department of Medical Genetics, College of Medicine, Hallym University, Chuncheon, Republic of Korea jgsuh@hallym.ac.kr zoonox@nate.com. 9. Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Hallym University, Chuncheon, Republic of Korea jgsuh@hallym.ac.kr zoonox@nate.com.
Abstract
BACKGROUND/AIM: The purpose of the present study was to delineate the cervical and facial vascular and associated anatomy in five murine species, and compare them for optimal use in research studies focused on understanding the pathology and treatment of diseases in humans. MATERIALS AND METHODS: The specific adult male animals examined were mice (C57BL/6J), rats (F344), mongolian gerbils (Merionesunguiculatus), hamsters (Syrian), and guinea pigs (Hartley). To stain the vasculature and organs, of the face and neck, each animal was systemically perfused using the vital stain, Trypan Blue. Following this step, the detailed anatomy of the head and neck could be easily visualized in all species. RESULTS: Unique morphological characteristics were demonstrated by comparing the five species, including symmetry of the common carotid origin bilaterally in the Mongolian Gerbil, a large submandibular gland in the hamster and an enlarged buccal branch in the Guinea Pig. In reviewing the anatomical details, this staining technique proves superior for direct surgical visualization and identification. CONCLUSION: The anatomical details provided through these five species atlas will help experimental researchers in the future to select the most appropriate animal model for specific laboratory studies aimed to improve our understanding and treatment of diseases in patients. Copyright
BACKGROUND/AIM: The purpose of the present study was to delineate the cervical and facial vascular and associated anatomy in five murine species, and compare them for optimal use in research studies focused on understanding the pathology and treatment of diseases in humans. MATERIALS AND METHODS: The specific adult male animals examined were mice (C57BL/6J), rats (F344), mongolian gerbils (Merionesunguiculatus), hamsters (Syrian), and guinea pigs (Hartley). To stain the vasculature and organs, of the face and neck, each animal was systemically perfused using the vital stain, Trypan Blue. Following this step, the detailed anatomy of the head and neck could be easily visualized in all species. RESULTS: Unique morphological characteristics were demonstrated by comparing the five species, including symmetry of the common carotid origin bilaterally in the Mongolian Gerbil, a large submandibular gland in the hamster and an enlarged buccal branch in the Guinea Pig. In reviewing the anatomical details, this staining technique proves superior for direct surgical visualization and identification. CONCLUSION: The anatomical details provided through these five species atlas will help experimental researchers in the future to select the most appropriate animal model for specific laboratory studies aimed to improve our understanding and treatment of diseases in patients. Copyright
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