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Literature DB >> 2888227

Bovine monoclonal antibodies to the F5 (K99) pilus antigen of E. coli, produced by murine/bovine hybridomas.

D V Anderson, E M Tucker, J R Powell, P Porter.

Abstract

Lymph node cells from calves immunized with purified pilus antigen of K99+ enterotoxigenic E. coli (ETEC) were fused with mouse myeloma (NSO) cells, and with non-Ig producing mouse/calf hybridomas or with a bovine Ig-producing mouse/calf/calf secondary hybridoma. Lines secreting bovine monoclonal IgG1 specific for K99 pilus antigen in an ELISA were obtained in each case. The two lines derived from xenohybridoma fusion partners have been secreting anti-K99 bovine monoclonal antibody for over one year in continual passage. None of the antibodies cross-reacted with other pilus types including K88, CFAI, CFAII, 987P or CP; they all inhibited agglutination of horse RBC (which have a K99 receptor) in the presence of K99 antigen; they showed positive fluorescence in an indirect binding assay on K99+ ETEC and inhibited K99+ ETEC adhesion to piglet enterocytes. These antibodies have potential prophylactic and therapeutic use in control and treatment of diarrhoea.

Entities: CellLine Disease Species

Mesh：

Substances：

Year: 1987 PMID： 2888227 PMCID： PMC7133685 DOI： 10.1016/0165-2427(87)90085-7

Source DB: PubMed Journal: Vet Immunol Immunopathol ISSN： 0165-2427 Impact factor: 2.046

20 in total

1. Chromosome preparations of leukocytes cultured from human peripheral blood.

Authors: P S MOORHEAD; P C NOWELL; W J MELLMAN; D M BATTIPS; D A HUNGERFORD
Journal: Exp Cell Res Date: 1960-09 Impact factor: 3.905

2. Further observations on Escherichia coli enterotoxins with particular regard to those produced by atypical piglet strains and by calf and lamb strains: the transmissible nature of these enterotoxins and of a K antigen possessed by calf and lamb strains.

Authors: H W Smith; M A Linggood
Journal: J Med Microbiol Date: 1972-05 Impact factor: 2.472

3. Intestinal epithelial cell surface membrane glycoprotein synthesis. I. An indicator of cellular differentiation.

Authors: M M Weiser
Journal: J Biol Chem Date: 1973-04-10 Impact factor: 5.157

Review 4. Host-specific fimbrial adhesins of noninvasive enterotoxigenic Escherichia coli strains.

Authors: W Gaastra; F K de Graaf
Journal: Microbiol Rev Date: 1982-06

5. Construction and testing of mouse--human heteromyelomas for human monoclonal antibody production.

Authors: N N Teng; K S Lam; F Calvo Riera; H S Kaplan
Journal: Proc Natl Acad Sci U S A Date: 1983-12 Impact factor: 11.205

6. Detection of the K99 antigen by means of agglutination and immunoelectrophoresis in Escherichia coli isolates from calves and its correlation with entertoxigenicity.

Authors: P A Guinée; W H Jansen; C M Agterberg
Journal: Infect Immun Date: 1976-05 Impact factor: 3.441

7. Specific bovine monoclonal antibody produced by a re-fused mouse/calf hybridoma.

Authors: E M Tucker; A R Dain; S W Clarke; R A Donker
Journal: Hybridoma Date: 1984

8. Protection of calves against fatal enteric colibacillosis by orally administered Escherichia coli K99-specific monoclonal antibody.

Authors: D M Sherman; S D Acres; P L Sadowski; J A Springer; B Bray; T J Raybould; C C Muscoplat
Journal: Infect Immun Date: 1983-11 Impact factor: 3.441

Review 4. Swine and cattle enterotoxigenic Escherichia coli-mediated diarrhea. Development of therapies based on inhibition of bacteria-host interactions.

Authors: M Mouricout
Journal: Eur J Epidemiol Date: 1991-11 Impact factor: 8.082

Review 5. Molecular Dissection of the Antibody Response: Opportunities and Needs for Application in Cattle.

Authors: Ruben Barroso; W Ivan Morrison; Liam J Morrison
Journal: Front Immunol Date: 2020-06-12 Impact factor: 7.561

5 in total

Bovine monoclonal antibodies to the F5 (K99) pilus antigen of E. coli, produced by murine/bovine hybridomas.

1. Chromosome preparations of leukocytes cultured from human peripheral blood.

2. Further observations on Escherichia coli enterotoxins with particular regard to those produced by atypical piglet strains and by calf and lamb strains: the transmissible nature of these enterotoxins and of a K antigen possessed by calf and lamb strains.

3. Intestinal epithelial cell surface membrane glycoprotein synthesis. I. An indicator of cellular differentiation.

Review 4. Host-specific fimbrial adhesins of noninvasive enterotoxigenic Escherichia coli strains.

5. Construction and testing of mouse--human heteromyelomas for human monoclonal antibody production.

6. Detection of the K99 antigen by means of agglutination and immunoelectrophoresis in Escherichia coli isolates from calves and its correlation with entertoxigenicity.

7. Specific bovine monoclonal antibody produced by a re-fused mouse/calf hybridoma.

8. Protection of calves against fatal enteric colibacillosis by orally administered Escherichia coli K99-specific monoclonal antibody.

9. Human-mouse hybrid cell lines containing partial complements of human chromosomes and functioning human genes.

10. Bovine x mouse hybridomas that secrete bovine immunoglobulin G1.

1. Bovine monoclonal antibody specific for Brucella abortus lipopolysaccharide.

2. A bovine monoclonal antibody to oestrone/oestradiol prepared by a (murine x bovine) xbovine interspecies fusion.

Review 3. The production and application of non-rodent monoclonal antibodies in veterinary science.

Review 4. Swine and cattle enterotoxigenic Escherichia coli-mediated diarrhea. Development of therapies based on inhibition of bacteria-host interactions.

Review 5. Molecular Dissection of the Antibody Response: Opportunities and Needs for Application in Cattle.