Najeeb Ur Rehman1, Liaqat Ali1,2, Ahmed Al-Harrasi1, Fazal Mabood3, Muhammed Al-Broumi3, Abdul Latif Khan1, Hidayat Hussain1, Javid Hussain3, René Csuk4. 1. UoN Chair of Oman's Medicinal Plants and Marine Natural Products, University of Nizwa, Nizwa, -616, Sultanate of Oman. 2. Department of Chemistry, University of Sargodha, Pakistan. 3. Department of Biological Sciences and Chemistry, College of Arts and Sciences, University of Nizwa, Nizwa-616, Sultanate of Oman. 4. Organic Chemistry, Martin-Luther-University Halle-Wittenberg, Kurt-Mothes-Str. 2, D-06120, Halle (Saale), Germany.
Abstract
INTRODUCTION: 3-O-Acetyl-11-keto-β-boswellic acid (AKBA), one of the pentacyclic triterpenoids, is the main biologically active constituent in the resin of Boswellia sacra and has received significant pharmacological interest in recent years. OBJECTIVE: It was aimed to develop a robust method to quantify the AKBA content in methanolic extracts of different parts of B. sacra plants and in various fractions of its resin exudates through near-infrared spectroscopy (NIRS) coupled with partial least squares regression (PLSR). MATERIAL AND METHODS: The near-infrared (NIR) spectra were used to measure the AKBA standards and B. sacra samples at a wavelength range between 700 and 2500 nm in absorption mode. A PLSR model was built from the obtained spectral data using 70% of the AKBA working standard solutions (training set), ranging from 0.1 ppm to 100 ppm. The final PLSR showed a R2 value of 99% with a root mean square error of cross-validation (RMSECV) value of 0.39% and a R2 value of 99%. RESULTS: The results showed that a 50% CHCl3 /n-hexane sub-fraction has the highest concentration of AKBA (14.8%), followed by 55% CHCl3 /n-hexane (13.6%), and 40% CHCl3 /n-hexane (6.1%). CONCLUSION: As the results achieved with the proposed NIRS methodology are in close agreement to the results of AKBA analysis using HPLC, we suggest that our proposed NIRS method is a fast alternative and non-destructive method for the analysis of AKBA in different samples of B. sacra.
INTRODUCTION: 3-O-Acetyl-11-keto-β-boswellic acid (AKBA), one of the pentacyclic triterpenoids, is the main biologically active constituent in the resin of Boswellia sacra and has received significant pharmacological interest in recent years. OBJECTIVE: It was aimed to develop a robust method to quantify the AKBA content in methanolic extracts of different parts of B. sacra plants and in various fractions of its resin exudates through near-infrared spectroscopy (NIRS) coupled with partial least squares regression (PLSR). MATERIAL AND METHODS: The near-infrared (NIR) spectra were used to measure the AKBA standards and B. sacra samples at a wavelength range between 700 and 2500 nm in absorption mode. A PLSR model was built from the obtained spectral data using 70% of the AKBA working standard solutions (training set), ranging from 0.1 ppm to 100 ppm. The final PLSR showed a R2 value of 99% with a root mean square error of cross-validation (RMSECV) value of 0.39% and a R2 value of 99%. RESULTS: The results showed that a 50% CHCl3 /n-hexane sub-fraction has the highest concentration of AKBA (14.8%), followed by 55% CHCl3 /n-hexane (13.6%), and 40% CHCl3 /n-hexane (6.1%). CONCLUSION: As the results achieved with the proposed NIRS methodology are in close agreement to the results of AKBA analysis using HPLC, we suggest that our proposed NIRS method is a fast alternative and non-destructive method for the analysis of AKBA in different samples of B. sacra.
Authors: Ahmed Al-Harrasi; Najeeb Ur Rehman; Abdul Latif Khan; Muhammed Al-Broumi; Issa Al-Amri; Javid Hussain; Hidayat Hussain; René Csuk Journal: PLoS One Date: 2018-06-18 Impact factor: 3.240