Arturas Sukovas1, Vaidotas Cesna2,3, Aldona Jasukaitiene3, Giedrius Barauskas2, Ruta Jolanta Nadisauskiene4, Zilvinas Dambrauskas3, Saulius Paskauskas4, Antanas Gulbinas3. 1. Department of Obstetrics and Gynecology, Lithuanian University of Health Sciences, Kaunas, Lithuania arturas.sukovas@lsmuni.lt arturas.sukovas@gmail.com. 2. Department of Surgery, Lithuanian University of Health Sciences, Kaunas, Lithuania. 3. Institute for Digestive Research, Lithuanian University of Health Sciences, Kaunas, Lithuania. 4. Department of Obstetrics and Gynecology, Lithuanian University of Health Sciences, Kaunas, Lithuania.
Abstract
BACKGROUND: Hyperthermic intraperitoneal chemotherapy (HIPEC) is proposed as a promising treatment method, but fundamental information about the contribution of hyperthermia to intraperitoneal chemotherapy is lacking. The purpose of this study was to investigate the cytotoxic effect of hyperthermia and cisplatin on OVCAR-3 cells in vitro. MATERIALS AND METHODS: Imitating the typical clinical conditions of HIPEC, OVCAR-3 cells were exposed to hyperthermia and cisplatin for 1 h. MTT viability test, flow cytometric analysis, and real-time cell and isobologram analysis were performed. RESULTS: Hyperthermia up to 42°C did not significantly increase the effect of cisplatin regarding the viability and apoptosis of OVCAR-3 cells. Moreover, an antagonistic effect of hyperthermia and cisplatin was revealed. CONCLUSION: Our investigation of OVCAR-3 cells critically disputes the benefit of hyperthermia in ovarian cancer treatment. Further in vitro and in vivo research is essential for better understanding of the mechanisms of action of hyperthermia and its role in the treatment of epithelial ovarian cancer. Copyright
BACKGROUND: Hyperthermic intraperitoneal chemotherapy (HIPEC) is proposed as a promising treatment method, but fundamental information about the contribution of hyperthermia to intraperitoneal chemotherapy is lacking. The purpose of this study was to investigate the cytotoxic effect of hyperthermia and cisplatin on OVCAR-3 cells in vitro. MATERIALS AND METHODS: Imitating the typical clinical conditions of HIPEC, OVCAR-3 cells were exposed to hyperthermia and cisplatin for 1 h. MTT viability test, flow cytometric analysis, and real-time cell and isobologram analysis were performed. RESULTS:Hyperthermia up to 42°C did not significantly increase the effect of cisplatin regarding the viability and apoptosis of OVCAR-3 cells. Moreover, an antagonistic effect of hyperthermia and cisplatin was revealed. CONCLUSION: Our investigation of OVCAR-3 cells critically disputes the benefit of hyperthermia in ovarian cancer treatment. Further in vitro and in vivo research is essential for better understanding of the mechanisms of action of hyperthermia and its role in the treatment of epithelial ovarian cancer. Copyright
Authors: Michelle Bilbao; Chelsea Katz; Stephanie L Kass; Devon Smith; Krystal Hunter; David Warshal; James K Aikins; Olga Ostrovsky Journal: Biomolecules Date: 2021-11-17