Fernando Salvador1, Elena Sulleiro2, Maria Piron3,4, Adrián Sánchez-Montalvá1, Silvia Sauleda3,4, Daniel Molina-Morant1, Zaira Moure2, Israel Molina1. 1. Department of Infectious Diseases, Vall d'Hebron University Hospital, PROSICS Barcelona, Universitat Autònoma de Barcelona, Barcelona, Spain. 2. Department of Microbiology, Vall d'Hebron University Hospital, PROSICS Barcelona, Barcelona, Spain. 3. Catalan Blood and Tissue Bank, Transfusion Safety Laboratory, Barcelona, Spain. 4. Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas, Instituto de Salud Carlos III, Madrid, Spain.
Abstract
OBJECTIVES: In a previous study performed by our group, Strongyloides stercoralis infection in patients with Chagas disease was associated with higher proportion of Trypanosoma cruzi DNA detection in peripheral blood. The aim of the study was to confirm this association in a larger cohort of patients. METHODS: Cross-sectional study of all patients with Chagas disease diagnosed from 2005 to 2015 during blood donation at the Catalan Blood Bank. Demographic data and T. cruzi RT-PCR were collected. S. stercoralis infection diagnosis was based on a serological test. RESULTS: Two hundred and two blood donors were included. T. cruzi RT-PCR was positive in 72 (35.6%) patients, and S. stercoralis serology was positive in 22 (10.9%) patients. Patients with positive S. stercoralis serology had higher proportion of positive T. cruzi RT-PCR than those with negative serology (54.5% vs. 33.3%, P = 0.050), and the difference increased when taking a serological index cut-off of 2.5, which increases the specificity of the test to detect a confirmed strongyloidiasis (60% vs. 33%, P = 0.017). CONCLUSIONS: Patients with Chagas disease with positive S. stercoralis serology had higher proportion of positive T. cruzi RT-PCR in peripheral blood than those with negative serology, which reflects the potential immunomodulatory effects of S. stercoralis in T. cruzi co-infected patients.
OBJECTIVES: In a previous study performed by our group, Strongyloides stercoralis infection in patients with Chagas disease was associated with higher proportion of Trypanosoma cruzi DNA detection in peripheral blood. The aim of the study was to confirm this association in a larger cohort of patients. METHODS: Cross-sectional study of all patients with Chagas disease diagnosed from 2005 to 2015 during blood donation at the Catalan Blood Bank. Demographic data and T. cruzi RT-PCR were collected. S. stercoralisinfection diagnosis was based on a serological test. RESULTS: Two hundred and two blood donors were included. T. cruzi RT-PCR was positive in 72 (35.6%) patients, and S. stercoralis serology was positive in 22 (10.9%) patients. Patients with positive S. stercoralis serology had higher proportion of positive T. cruzi RT-PCR than those with negative serology (54.5% vs. 33.3%, P = 0.050), and the difference increased when taking a serological index cut-off of 2.5, which increases the specificity of the test to detect a confirmed strongyloidiasis (60% vs. 33%, P = 0.017). CONCLUSIONS:Patients with Chagas disease with positive S. stercoralis serology had higher proportion of positive T. cruzi RT-PCR in peripheral blood than those with negative serology, which reflects the potential immunomodulatory effects of S. stercoralis in T. cruzi co-infected patients.
Authors: Fernando Salvador; Adrián Sánchez-Montalvá; Mónica Martínez-Gallo; Elena Sulleiro; Clara Franco-Jarava; Augusto Sao Avilés; Pau Bosch-Nicolau; Zaira Moure; Aroa Silgado; Israel Molina Journal: Am J Trop Med Hyg Date: 2020-01 Impact factor: 2.345
Authors: Fernando Salvador; Begoña Treviño; Sandra Chamorro-Tojeiro; Adrián Sánchez-Montalvá; Juan María Herrero-Martínez; Azucena Rodríguez-Guardado; Núria Serre-Delcor; Diego Torrús; Josune Goikoetxea; Zuriñe Zubero; María Velasco; Elena Sulleiro; Israel Molina; Rogelio López-Vélez; José Antonio Pérez-Molina Journal: PLoS Negl Trop Dis Date: 2019-05-16
Authors: Melissa S Nolan; Kristy O Murray; Rojelio Mejia; Peter J Hotez; Maria Jose Villar Mondragon; Stanley Rodriguez; Jose Ricardo Palacios; William Ernesto Murcia Contreras; M Katie Lynn; Myriam E Torres; Maria Carlota Monroy Escobar Journal: Trop Med Infect Dis Date: 2021-05-07