Line Staun Larsen1, Vibeke Baelum2, Livia Maria Andaló Tenuta3, Alan Richards4, Bente Nyvad4. 1. Section of Dental Pathology, Operative Dentistry and Endodontics, Department of Dentistry and Oral Health, HEALTH, Aarhus University, Vennelyst Boulevard 9, 8000, Aarhus C, Denmark. line.staun@dent.au.dk. 2. Section of Oral Epidemiology and Dental Public Health, Department of Dentistry and Oral Health, HEALTH, Aarhus University, Vennelyst Boulevard 9, 8000, Aarhus C, Denmark. 3. Department of Physiological Sciences, Piracicaba Dental School, UNICAMP, Av. Limeira 901, Piracicaba, São Paulo, 13414-903, Brazil. 4. Section of Dental Pathology, Operative Dentistry and Endodontics, Department of Dentistry and Oral Health, HEALTH, Aarhus University, Vennelyst Boulevard 9, 8000, Aarhus C, Denmark.
Abstract
OBJECTIVES: The aim of this randomized, double-blind, crossover study was to measure fluoride in saliva and 7-day-old biofilm fluid and biofilm solids after rinsing three times per day for 3 weeks with 0, 1500, or 5000 ppm fluoride (NaF). MATERIALS AND METHODS: Following the 3-week wash-in/wash-out period, including 1 week of biofilm accumulation, saliva and biofilm samples were collected from 12 participants immediately before (background fluoride), and 10, 30, and 60 min after a single rinse. Biofilm samples were separated into fluid and solids, and samples were analyzed using a fluoride electrode (microanalysis). RESULTS: The background fluoride concentration was statistically significantly higher in the 5000 compared to the 1500 ppm F rinse group in all three compartments (22.3 and 8.1 μM in saliva, 126.8 and 58.5 μM in biofilm fluid, and 10,940 and 4837 μmol/kg in biofilm solids). The 1-h fluoride accumulation for the 5000 ppm F rinse was higher than for the 1500 ppm F rinse in all three compartments, although not statistically significant for saliva and biofilm solids. CONCLUSION:Regular exposure to 5000 ppm fluoride elevates background fluoride concentrations in saliva, biofilm fluid, and biofilm solids compared to 1500 ppm fluoride. Increasing the fluoride concentration almost 3.5 times (from 1500 to 5000 ppm) only elevates the background fluoride concentrations in saliva, biofilm fluid, and biofilm solids twofold. CLINICAL RELEVANCE: Even though fluoride toothpaste may be diluted by saliva, the results of the present study indicate that use of 5000 ppm fluoride toothpaste might lead to improved caries control.
RCT Entities:
OBJECTIVES: The aim of this randomized, double-blind, crossover study was to measure fluoride in saliva and 7-day-old biofilm fluid and biofilm solids after rinsing three times per day for 3 weeks with 0, 1500, or 5000 ppm fluoride (NaF). MATERIALS AND METHODS: Following the 3-week wash-in/wash-out period, including 1 week of biofilm accumulation, saliva and biofilm samples were collected from 12 participants immediately before (background fluoride), and 10, 30, and 60 min after a single rinse. Biofilm samples were separated into fluid and solids, and samples were analyzed using a fluoride electrode (microanalysis). RESULTS: The background fluoride concentration was statistically significantly higher in the 5000 compared to the 1500 ppm F rinse group in all three compartments (22.3 and 8.1 μM in saliva, 126.8 and 58.5 μM in biofilm fluid, and 10,940 and 4837 μmol/kg in biofilm solids). The 1-h fluoride accumulation for the 5000 ppm F rinse was higher than for the 1500 ppm F rinse in all three compartments, although not statistically significant for saliva and biofilm solids. CONCLUSION: Regular exposure to 5000 ppm fluoride elevates background fluoride concentrations in saliva, biofilm fluid, and biofilm solids compared to 1500 ppm fluoride. Increasing the fluoride concentration almost 3.5 times (from 1500 to 5000 ppm) only elevates the background fluoride concentrations in saliva, biofilm fluid, and biofilm solids twofold. CLINICAL RELEVANCE: Even though fluoride toothpaste may be diluted by saliva, the results of the present study indicate that use of 5000 ppm fluoride toothpaste might lead to improved caries control.
Authors: Francisco Josimar Girão Júnior; Lidia Audrey Rocha Valadas; Peter Bottenberg; Mara Assef Leitão Lotif; Edilson Martins Rodrigues Neto; Said Gonçalves da Cruz Fonseca; Mary Anne Medeiros Bandeira; Aldo Squassi; Thereza Cristina Farias Botelho Dantas; Nara Juliana Custódio de Sena; Marta Maria de França Fonteles Journal: Evid Based Complement Alternat Med Date: 2022-01-31 Impact factor: 2.629