Literature DB >> 28865052

An improved method for identifying SUMOylation sites of viral proteins.

Yan Liu1, Zhongyuan Tan1, Bo Shu1, Yuan Zhang1, Caishang Zheng1, Xianliang Ke1, Xi Chen2, Hanzhong Wang1, Zhenhua Zheng3.   

Abstract

In this study, we improved the most commonly used methods for MS detection of SUMOylated sites and used an E. coli recombination SUMOylation system with SUMO-1 (T95R). This system provides fast enrichment of SUMOylated viral protein in less than 2 days, and shows advantage over the method of collecting modified protein from cells in convenience and sensitivity. Furthermore, this method provides an option for rapid and accurate identification of the potential viral protein SUMOylation sites.

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Year:  2017        PMID： 28865052      PMCID： PMC6598889          DOI： 10.1007/s12250-017-4018-3

Source DB:  PubMed          Journal:  Virol Sin        ISSN： 1995-820X            Impact factor:   4.327


  16 in total

1.  Identification of endogenous SUMO1 accepter sites by mass spectrometry.

Authors:  He-Hsuan Hsiao; Erik Meulmeester; Henning Urlaub
Journal:  Methods Mol Biol       Date:  2012

2.  A universal strategy for proteomic studies of SUMO and other ubiquitin-like modifiers.

Authors:  Germán Rosas-Acosta; William K Russell; Adeline Deyrieux; David H Russell; Van G Wilson
Journal:  Mol Cell Proteomics       Date:  2004-11-30       Impact factor: 5.911

3.  SUMO Modification Stabilizes Enterovirus 71 Polymerase 3D To Facilitate Viral Replication.

Authors:  Yan Liu; Zhenhua Zheng; Bo Shu; Jin Meng; Yuan Zhang; Caishang Zheng; Xianliang Ke; Peng Gong; Qinxue Hu; Hanzhong Wang
Journal:  J Virol       Date:  2016-11-14       Impact factor: 5.103

4.  Mapping of SUMO sites and analysis of SUMOylation changes induced by external stimuli.

Authors:  Francis Impens; Lilliana Radoshevich; Pascale Cossart; David Ribet
Journal:  Proc Natl Acad Sci U S A       Date:  2014-08-11       Impact factor: 11.205

5.  Site-specific identification of SUMO-2 targets in cells reveals an inverted SUMOylation motif and a hydrophobic cluster SUMOylation motif.

Authors:  Ivan Matic; Joost Schimmel; Ivo A Hendriks; Maria A van Santen; Frans van de Rijke; Hans van Dam; Florian Gnad; Matthias Mann; Alfred C O Vertegaal
Journal:  Mol Cell       Date:  2010-08-27       Impact factor: 17.970

6.  Reconstitution of Arabidopsis thaliana SUMO pathways in E. coli: functional evaluation of SUMO machinery proteins and mapping of SUMOylation sites by mass spectrometry.

Authors:  Sachiko Okada; Mio Nagabuchi; Yusuke Takamura; Tsuyoshi Nakagawa; Kaori Shinmyozu; Jun-ichi Nakayama; Katsunori Tanaka
Journal:  Plant Cell Physiol       Date:  2009-04-17       Impact factor: 4.927

7.  A method of mapping protein sumoylation sites by mass spectrometry using a modified small ubiquitin-like modifier 1 (SUMO-1) and a computational program.

Authors:  Matthew Knuesel; Hiu Tom Cheung; Micah Hamady; Kristen K B Barthel; Xuedong Liu
Journal:  Mol Cell Proteomics       Date:  2005-07-14       Impact factor: 5.911

8.  The human cytomegalovirus DNA polymerase processivity factor UL44 is modified by SUMO in a DNA-dependent manner.

Authors:  Elisa Sinigalia; Gualtiero Alvisi; Chiara V Segré; Beatrice Mercorelli; Giulia Muratore; Michael Winkler; He-Hsuan Hsiao; Henning Urlaub; Alessandro Ripalti; Susanna Chiocca; Giorgio Palù; Arianna Loregian
Journal:  PLoS One       Date:  2012-11-15       Impact factor: 3.240

9.  In vivo identification of human small ubiquitin-like modifier polymerization sites by high accuracy mass spectrometry and an in vitro to in vivo strategy.

Authors:  Ivan Matic; Martijn van Hagen; Joost Schimmel; Boris Macek; Stephen C Ogg; Michael H Tatham; Ronald T Hay; Angus I Lamond; Matthias Mann; Alfred C O Vertegaal
Journal:  Mol Cell Proteomics       Date:  2007-10-15       Impact factor: 5.911

Review 10.  Viral Mimicry to Usurp Ubiquitin and SUMO Host Pathways.

Authors:  Peter Wimmer; Sabrina Schreiner
Journal:  Viruses       Date:  2015-08-28       Impact factor: 5.048
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  1 in total

Review 1.  MS-based strategies for identification of protein SUMOylation modification.

Authors:  Zenghua Sheng; Xixi Wang; Yanni Ma; Dan Zhang; Yanfang Yang; Peng Zhang; Hongxia Zhu; Ningzhi Xu; Shufang Liang
Journal:  Electrophoresis       Date:  2019-06-27       Impact factor: 3.535
  1 in total

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