Yunzi Long1, Siyi Liu1, Lin Zhu1, Qiming Liang2, Xiaofeng Chen2, Yanmei Dong3. 1. Department of Cariology and Endodontology, Peking University School and Hospital of Stomatology, Haidian District, Beijing, China. 2. National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, China. 3. Department of Cariology and Endodontology, Peking University School and Hospital of Stomatology, Haidian District, Beijing, China. Electronic address: kqdongyanmei@bjmu.edu.cn.
Abstract
INTRODUCTION: This study aimed to investigate dental pulp responses to novel bioactive glass (BG) pulp capping materials after direct pulp capping in vivo. METHODS: Novel BG pulp capping materials are composed of powder and fluid. The powder is BG (82.36% SiO2, 15.36% CaO, and 2.28% P2O5), and the fluid is provided in 2 kinds: (1) phosphate buffer solution (BG-PB) and (2) phosphate buffer solution with the addition of 1 wt% sodium alginate (BG-PB-SA). After mixing the powder and fluid, BG-PB and BG-PB-SA were prepared. Cavities with mechanical pulp exposure were prepared on maxillary first molars of Wistar rats. The exposures were randomly capped with BG-PB, BG-PB-SA, or mineral trioxide aggregate (MTA). After 1 (n = 6) and 4 weeks (n = 8), maxillary segments were obtained and prepared for histologic analysis with a scoring system. Statistical analysis was performed using the Kruskal-Wallis and Mann-Whitney U tests with the significance set at .05. RESULTS: After 1 week, few inflammatory cells were present in the BG-PB, BG-PB-SA, and MTA groups. Moreover, a thin layer of newly generated matrix was observed in most specimens. After 4 weeks, all specimens from the 3 groups formed a heavy dentin bridge. BG-PB and BG-PB-SA groups exhibited no or slight inflammatory response, whereas the MTA group exhibited a slight to moderate inflammatory response. No significant difference was observed in pulp inflammation and dentin formation among the 3 groups at either time point (P > .05). CONCLUSIONS: When used as a pulp capping agent, BG-PB and BG-PB-SA had similar favorable cellular and inflammatory pulp responses to those of MTA. Therefore, BG is a promising pulp capping material.
INTRODUCTION: This study aimed to investigate dental pulp responses to novel bioactive glass (BG) pulp capping materials after direct pulp capping in vivo. METHODS: Novel BG pulp capping materials are composed of powder and fluid. The powder is BG (82.36% SiO2, 15.36% CaO, and 2.28% P2O5), and the fluid is provided in 2 kinds: (1) phosphate buffer solution (BG-PB) and (2) phosphate buffer solution with the addition of 1 wt% sodium alginate (BG-PB-SA). After mixing the powder and fluid, BG-PB and BG-PB-SA were prepared. Cavities with mechanical pulp exposure were prepared on maxillary first molars of Wistar rats. The exposures were randomly capped with BG-PB, BG-PB-SA, or mineral trioxide aggregate (MTA). After 1 (n = 6) and 4 weeks (n = 8), maxillary segments were obtained and prepared for histologic analysis with a scoring system. Statistical analysis was performed using the Kruskal-Wallis and Mann-Whitney U tests with the significance set at .05. RESULTS: After 1 week, few inflammatory cells were present in the BG-PB, BG-PB-SA, and MTA groups. Moreover, a thin layer of newly generated matrix was observed in most specimens. After 4 weeks, all specimens from the 3 groups formed a heavy dentin bridge. BG-PB and BG-PB-SA groups exhibited no or slight inflammatory response, whereas the MTA group exhibited a slight to moderate inflammatory response. No significant difference was observed in pulp inflammation and dentin formation among the 3 groups at either time point (P > .05). CONCLUSIONS: When used as a pulp capping agent, BG-PB and BG-PB-SA had similar favorable cellular and inflammatory pulp responses to those of MTA. Therefore, BG is a promising pulp capping material.
Authors: Yasmine Elhamouly; Rania M El Backly; Dalia M Talaat; Samia S Omar; Maha El Tantawi; Karin M L Dowidar Journal: Clin Oral Investig Date: 2021-01-06 Impact factor: 3.573