Literature DB >> 2886389

ATP-binding properties of P glycoprotein from multidrug-resistant KB cells.

M M Cornwell, T Tsuruo, M M Gottesman, I Pastan.   

Abstract

The photoaffinity reagent 8-azido-alpha-[32P]ATP was used to label a protein of 170 kDa in membrane vesicle preparations from a highly multidrug-resistant cell line, KB-V1, but not from the drug-sensitive parental cell line KB-3-1. The 170-kDa labeled protein was immunoprecipitated with a monoclonal antibody (MRK-16) to P glycoprotein. Both ATP and GTP inhibited labeling by 8-azido-alpha-[32P]ATP. Labeling of P170 was not inhibited by 5 mM ADP, 5 mM ribose-5-phosphate, or 100 microM vinblastine. These data directly demonstrate that P glycoprotein has a nucleotide-binding site that could supply energy for drug transport.

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Year:  1987        PMID： 2886389     DOI： 10.1096/fasebj.1.1.2886389

Source DB:  PubMed          Journal:  FASEB J        ISSN： 0892-6638            Impact factor:   5.191


  34 in total

Review 1.  Improving the prediction of the brain disposition for orally administered drugs using BDDCS.

Authors:  Fabio Broccatelli; Caroline A Larregieu; Gabriele Cruciani; Tudor I Oprea; Leslie Z Benet
Journal:  Adv Drug Deliv Rev       Date:  2011-12-21       Impact factor: 15.470

2.  A single amino acid substitution strongly modulates the activity and substrate specificity of the mouse mdr1 and mdr3 drug efflux pumps.

Authors:  P Gros; R Dhir; J Croop; F Talbot
Journal:  Proc Natl Acad Sci U S A       Date:  1991-08-15       Impact factor: 11.205

3.  Functional expression of mouse Mdr1 in an outer membrane permeability mutant of Escherichia coli.

Authors:  O Béjà; E Bibi
Journal:  Proc Natl Acad Sci U S A       Date:  1996-06-11       Impact factor: 11.205

4.  Dual modulation of chloride conductance by nucleotides in pancreatic and parotid zymogen granules.

Authors:  F Thévenod; K W Gasser; U Hopfer
Journal:  Biochem J       Date:  1990-11-15       Impact factor: 3.857

5.  Functional analysis of chimeric genes obtained by exchanging homologous domains of the mouse mdr1 and mdr2 genes.

Authors:  E Buschman; P Gros
Journal:  Mol Cell Biol       Date:  1991-02       Impact factor: 4.272

6.  Photoaffinity labeling of the multidrug-resistance-related P-glycoprotein with photoactive analogs of verapamil.

Authors:  A R Safa
Journal:  Proc Natl Acad Sci U S A       Date:  1988-10       Impact factor: 11.205

7.  ATP-dependent transport of vinblastine in vesicles from human multidrug-resistant cells.

Authors:  M Horio; M M Gottesman; I Pastan
Journal:  Proc Natl Acad Sci U S A       Date:  1988-05       Impact factor: 11.205

8.  Magnesium Adenosine 5[prime]-Triphosphate-Energized Transport of Glutathione-S-Conjugates by Plant Vacuolar Membrane Vesicles.

Authors:  Z. S. Li; Y. Zhao; P. A. Rea
Journal:  Plant Physiol       Date:  1995-04       Impact factor: 8.340

9.  Functional expression of mouse mdr1 in Escherichia coli.

Authors:  E Bibi; P Gros; H R Kaback
Journal:  Proc Natl Acad Sci U S A       Date:  1993-10-01       Impact factor: 11.205

10.  Partial purification and reconstitution of the human multidrug-resistance pump: characterization of the drug-stimulatable ATP hydrolysis.

Authors:  S V Ambudkar; I H Lelong; J Zhang; C O Cardarelli; M M Gottesman; I Pastan
Journal:  Proc Natl Acad Sci U S A       Date:  1992-09-15       Impact factor: 11.205
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