The status of the study of invertebrate neurons in tissue culture--phylum Arthropoda.

1. Neurobiological studies employing tissue culture are discussed for all invertebrate organisms in phylum Arthropoda and above. Only arthropod species have been investigated in these studies. 2. The members of phylum Arthropoda utilized were Periplaneta, Locusta, Drosophila, and Schistocerca. 3. Some of the initial studies of nerve cells in tissue culture were performed with Periplaneta and Drosophila; these studies were primarily concerned with differentiation, growth and development. 4. Cultured neurons from cockroach and locust have been used in pharmacological investigations of neurotransmitter receptors. 5. Embryos of the grasshopper Schistocerca were cultured in series of developmental studies related to axon guidance and peripheral nerve formation. 6. Recent studies with Drosophila neurons in vitro have applied a genetic approach to the investigation of molecular properties and function of the axonal sodium channel. 7. An overall view of neuronal tissue culture of invertebrates and analysis of the various areas of research is presented.