Expression of Gene Encoding Endo-1,4-β-glucanase in Suspension-cultured Poplar (Poplus alba L.) Cells†.

The level of mRNA for endo-1,4-β-glucanase was increased before the exponential phase of growth and decreased rapidly during the exponential phase in suspension-cultured poplar cells. The level of mRNA was increased for a short period after the addition of either 2,4-D or sucrose to the culture medium at the stationary phase. The level was increased to the maximal level when both 2,4-D and sucrose were provided together, but one did not increase the effect of the other. These findings suggest that expression of gene encoding poplar endo-1,4-β-glucanase is controlled during cell growth by independent systems activated by auxin and sucrose.