Ping Zhao1,2, Donna Wendt3, Sean Z Goodin3, Shwetha Ravichandran3, Tara E Chouinard4, April D Strader3. 1. Department of Physiology, Southern Illinois University School of Medicine, Carbondale, IL, 62901, USA. pzhao1@una.edu. 2. Department of Biology, University of North Alabama, 1 Harrison Plaza, Florence, AL, 35632, USA. pzhao1@una.edu. 3. Department of Physiology, Southern Illinois University School of Medicine, Carbondale, IL, 62901, USA. 4. Endocrine, Cardio Metabolic Disease DHT, Eli Lilly and Company, Indianapolis, IN, 46285, USA.
Abstract
PURPOSE: Ileal interposition recapitulates many of the metabolic improvements similar to Roux-en-Y gastric bypass. We aimed to determine whether the metabolic improvements seen following ileal interposition were conferred solely by the interposed segment by examining changes in neighboring intestinal segments as well as the composition of the bile acid pool. MATERIALS AND METHODS: Adult male rats were treated with either sham or ileal interposition surgeries. Glucose tolerance tests, body composition analysis, polymer chain reaction, enzyme-linked immunosorbent assay, and mass spectrometry were done after the surgeries. RESULTS: This study showed that ileal interposition improved glucose tolerance and enhanced both fasting and glucose-stimulated GLP-1 secretion in diabetic rats. Total bile acid pool was similar between groups but the composition favored glycine-conjugation in rats with ileal interposition. Insulin secretion was highly correlated with the 12-alpha-hydroxylase index of activity. The interposed ileum exhibited an increase in mRNA for preproglucagon and peptide YY; however, the bile acid transporter, apical sodium bile acid transporter, was dramatically reduced compared to sham rats. The interposed segment becomes jejunized in its new location as indicated by an increase in Glut2 and Pepck mRNA, genes predominantly synthesized within the jejunum. CONCLUSION: Ileal relocation alone can significantly alter the bile acid pool to favor a more insulin-sensitive metabolism in association with intestinal wide alterations in mRNA for a variety of genes. Ileal interposition may confer metabolic improvement via both the interposed segment and the associated intestinal changes in all segments of the intestine, including the colon.
PURPOSE: Ileal interposition recapitulates many of the metabolic improvements similar to Roux-en-Y gastric bypass. We aimed to determine whether the metabolic improvements seen following ileal interposition were conferred solely by the interposed segment by examining changes in neighboring intestinal segments as well as the composition of the bile acid pool. MATERIALS AND METHODS: Adult male rats were treated with either sham or ileal interposition surgeries. Glucose tolerance tests, body composition analysis, polymer chain reaction, enzyme-linked immunosorbent assay, and mass spectrometry were done after the surgeries. RESULTS: This study showed that ileal interposition improved glucose tolerance and enhanced both fasting and glucose-stimulated GLP-1 secretion in diabeticrats. Total bile acid pool was similar between groups but the composition favored glycine-conjugation in rats with ileal interposition. Insulin secretion was highly correlated with the 12-alpha-hydroxylase index of activity. The interposed ileum exhibited an increase in mRNA for preproglucagon and peptide YY; however, the bile acid transporter, apical sodium bile acid transporter, was dramatically reduced compared to sham rats. The interposed segment becomes jejunized in its new location as indicated by an increase in Glut2 and Pepck mRNA, genes predominantly synthesized within the jejunum. CONCLUSION: Ileal relocation alone can significantly alter the bile acid pool to favor a more insulin-sensitive metabolism in association with intestinal wide alterations in mRNA for a variety of genes. Ileal interposition may confer metabolic improvement via both the interposed segment and the associated intestinal changes in all segments of the intestine, including the colon.
Entities:
Keywords:
Intestine and bile changes after ileal interposition
Authors: Tiangang Li; Jessica M Francl; Shannon Boehme; Adrian Ochoa; Youcai Zhang; Curtis D Klaassen; Sandra K Erickson; John Y L Chiang Journal: J Biol Chem Date: 2011-12-05 Impact factor: 5.157
Authors: Achint Kaur; Jay V Patankar; Willeke de Haan; Piers Ruddle; Nadeeja Wijesekara; Albert K Groen; C Bruce Verchere; Roshni R Singaraja; Michael R Hayden Journal: Diabetes Date: 2014-10-22 Impact factor: 9.461
Authors: Brynn S Wolff; Katia Meirelles; Qinghe Meng; Ming Pan; Robert N Cooney Journal: Am J Physiol Gastrointest Liver Physiol Date: 2009-06-25 Impact factor: 4.052
Authors: T Tsuchiya; J Ishizuka; K Sato; I Shimoda; S Rajaraman; T Uchida; C M Townsend; J C Thompson Journal: J Gerontol A Biol Sci Med Sci Date: 1995-05 Impact factor: 6.053
Authors: Patrick J Antonellis; Brian A Droz; Richard Cosgrove; Libbey S O'Farrell; Tamer Coskun; James W Perfield; Steven Bauer; Mark Wade; Tara E Chouinard; Joseph T Brozinick; Andrew C Adams; Ricardo J Samms Journal: Mol Metab Date: 2019-09-29 Impact factor: 7.422