Hailong Hao1,2, Lei Wang3, Huiqing Chen2, Liwu Xie4, Tao Bai5, Hongyu Liu2, Dongwen Wang1. 1. Department of Urology, First Hospital of Shanxi Medical UniversityTaiyuan 030001, Shanxi, China. 2. Department of Urology, Shanxi Provincial Cancer HospitalTaiyuan 030013, Shanxi, China. 3. Department of Respiratory Medicine, Shanxi Dayi HospitalTaiyuan 030013, Shanxi, China. 4. Department of Pathology, Shanxi Provincial Cancer HospitalTaiyuan 030013, Shanxi, China. 5. Department of Pathology, First Hospital of Shanxi Medical UniversityTaiyuan 030001, Shanxi, China.
Abstract
OBJECTIVE: This study aims to observe the expression of YKL-40 in prostate cancer and whether YKL-40 can affect the migration and invasion of tumor cells by regulating epithelial mesenchymal transition. METHODS: We collected 14 cases of prostate cancer tissues and adjacent tissues in this study. The expression levels of YKL-40 in the tissues were analyzed by western blotting and immunohistochemical methods. The expression of YKL-40 in human prostate cancer cell line DU145 and PC3 was detected by fluorescence quantitative PCR and western blotting methods. The expression levels of YKL-40 in different cells were up-regulated or down- regulated by lentivirus to observe the changes of cell migration and invasion. The expression levels of EMT related genes were analyzed by RT-PCR and Western blotting methods. RESULTS: The expression level of YKL-40 in prostate cancer tissues was significantly higher than that in adjacent tissues (P<0.01), and it was higher in DU145 cells than that in PC3 cells (P<0.05). The expression level of YKL-40 was positively correlated with cell migration and invasion. YKL-40 can regulate the expression of EMT related genes (Twist, Snail, Slug, N-cadherin, Vimentin and E-cadherin). CONCLUSIONS: The expression level of YKL-40 was positively correlated with the migration and invasion of prostate cells, it affects cancer metastasis by regulating EMT.
OBJECTIVE: This study aims to observe the expression of YKL-40 in prostate cancer and whether YKL-40 can affect the migration and invasion of tumor cells by regulating epithelial mesenchymal transition. METHODS: We collected 14 cases of prostate cancer tissues and adjacent tissues in this study. The expression levels of YKL-40 in the tissues were analyzed by western blotting and immunohistochemical methods. The expression of YKL-40 in humanprostate cancer cell line DU145 and PC3 was detected by fluorescence quantitative PCR and western blotting methods. The expression levels of YKL-40 in different cells were up-regulated or down- regulated by lentivirus to observe the changes of cell migration and invasion. The expression levels of EMT related genes were analyzed by RT-PCR and Western blotting methods. RESULTS: The expression level of YKL-40 in prostate cancer tissues was significantly higher than that in adjacent tissues (P<0.01), and it was higher in DU145 cells than that in PC3 cells (P<0.05). The expression level of YKL-40 was positively correlated with cell migration and invasion. YKL-40 can regulate the expression of EMT related genes (Twist, Snail, Slug, N-cadherin, Vimentin and E-cadherin). CONCLUSIONS: The expression level of YKL-40 was positively correlated with the migration and invasion of prostate cells, it affects cancer metastasis by regulating EMT.
Authors: Bo Mi Ku; Yeon Kyung Lee; Jinhyun Ryu; Joo Yeon Jeong; Jungil Choi; Keyoung Mi Eun; Hye Young Shin; Dong Gyu Kim; Eun Mi Hwang; Jae Cheal Yoo; Jae-Yong Park; Gu Seob Roh; Hyun Joon Kim; Gyeong Jae Cho; Wan Sung Choi; Sun Ha Paek; Sang Soo Kang Journal: Int J Cancer Date: 2011-03-15 Impact factor: 7.396
Authors: Tarec C El-Galaly; Anders E Bilgrau; Eva Gaarsdal; Tobias W Klausen; Lars M Pedersen; Kaspar R Nielsen; John Bæch; Martin Bøgsted; Karen Dybkær; Julia S Johansen; Hans E Johnsen Journal: Leuk Lymphoma Date: 2015-02-24
Authors: Ralph A Francescone; Steve Scully; Michael Faibish; Sherry L Taylor; Dennis Oh; Luis Moral; Wei Yan; Brooke Bentley; Rong Shao Journal: J Biol Chem Date: 2011-03-08 Impact factor: 5.157