Lei Qiao1, Xiangyu Liu1, Yichao Tang1, Zheng Zhao1, Jilong Zhang1, Yong Feng2. 1. Department of Colorectal and Hernia Minimally Invasive Surgery, Shengjing Hospital of China Medical University, Shenyang 110004, People's Republic of China. 2. Department of Colorectal and Hernia Minimally Invasive Surgery, Shengjing Hospital of China Medical University, Shenyang 110004, People's Republic of China. Electronic address: fengyong_cmu2h@163.com.
Abstract
AIMS: The long non-coding RNA (lncRNA) was reported to be involved in the progress of various cancers, however, its effect in colorectal cancer (CRC) remains unknown. The goal of the present study is to investigate the function role of lncRNA PCAT-1 in colorectal cancer. MAIN METHODS: The expression of lncRNA PCAT-1 in four CRC cell lines was measured by real-time PCR, and two lncRNA PCAT-1 high expression cell lines were selected. LncRNA PCAT-1 in these two CRC cell lines was down-regulated by shRNA, and the stable transfected cells were established. Functional involvement of lncRNA PCAT-1 in proliferation and apoptosis of the two CRC cells were evaluated in vitro. Mover, the effect of lncRNA PCAT-1 in tumor proliferation was also evaluated in CRC cell xenograft. KEY FINDINGS: The results showed that down-regulation of lncRNA PCAT-1 in CRC cells inhibited proliferation, blocked cell cycle transition, and suppressed the expression of cyclins and c-myc. The apoptosis cell proportion was elevated with increased expression of pro-apoptotic proteins and decreased anti-apoptotic proteins in lncRNA PCAT-1 knock down cells. Forced over-expression of c-myc in PCAT-1 down-regulated CRC cells increased the level of cyclins. The xenograft growth in lncRNA PCAT-1 down-regulated cells was significantly inhibited along with the reduced proliferative cells. SIGNIFICANCE: Our study revealed a tumorigenic effect of lncRNA PCAT-1 in CRC cells, and this effect is partly dependent on the inhibition of c-myc.
AIMS: The long non-coding RNA (lncRNA) was reported to be involved in the progress of various cancers, however, its effect in colorectal cancer (CRC) remains unknown. The goal of the present study is to investigate the function role of lncRNA PCAT-1 in colorectal cancer. MAIN METHODS: The expression of lncRNA PCAT-1 in four CRC cell lines was measured by real-time PCR, and two lncRNA PCAT-1 high expression cell lines were selected. LncRNA PCAT-1 in these two CRC cell lines was down-regulated by shRNA, and the stable transfected cells were established. Functional involvement of lncRNA PCAT-1 in proliferation and apoptosis of the two CRC cells were evaluated in vitro. Mover, the effect of lncRNA PCAT-1 in tumor proliferation was also evaluated in CRC cell xenograft. KEY FINDINGS: The results showed that down-regulation of lncRNA PCAT-1 in CRC cells inhibited proliferation, blocked cell cycle transition, and suppressed the expression of cyclins and c-myc. The apoptosis cell proportion was elevated with increased expression of pro-apoptotic proteins and decreased anti-apoptotic proteins in lncRNA PCAT-1 knock down cells. Forced over-expression of c-myc in PCAT-1 down-regulated CRC cells increased the level of cyclins. The xenograft growth in lncRNA PCAT-1 down-regulated cells was significantly inhibited along with the reduced proliferative cells. SIGNIFICANCE: Our study revealed a tumorigenic effect of lncRNA PCAT-1 in CRC cells, and this effect is partly dependent on the inhibition of c-myc.
Authors: Muhammad Irfan; Zeeshan Javed; Khushbukhat Khan; Naila Khan; Anca Oana Docea; Daniela Calina; Javad Sharifi-Rad; William C Cho Journal: Cancer Cell Int Date: 2022-09-08 Impact factor: 6.429
Authors: Jana-Aletta Thiele; Petr Hosek; Eva Kralovcova; Pavel Ostasov; Vaclav Liska; Jan Bruha; Ondrej Vycital; Jachym Rosendorf; Alena Opattova; Josef Horak; Milena Kralickova; Pavel Vodicka; Pavel Pitule Journal: Int J Mol Sci Date: 2018-09-08 Impact factor: 5.923