Ye Yuan1, Gege Yan1, Rui Gong1, Lai Zhang1, Tianyi Liu1, Chao Feng1, Weijie Du1, Ying Wang1, Fan Yang1, Yuan Li1, Shuyuan Guo2, Fengzhi Ding1, Wenya Ma1, Elina Idiiatullina1,3, Valentin Pavlov3, Zhenbo Han1, Benzhi Cai1, Lei Yang4. 1. Department of Clinical Pharmacy, The Second Affiliated Hospital, and Department of Pharmacology, College of Pharmacy, Harbin Medical University, Harbin, China. 2. Department of Cardiology, the First Affiliated Hospital, Cardiovascular Institute, Harbin Medical University, Harbin, China. 3. Central Laboratory of Scientific Research, Bashkir State Medical University, Ufa, Russian Federation. 4. Department of Orthopedics, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
Abstract
BACKGROUND/AIMS: Blue light emitting diodes (LEDs) have been proven to affect the growth of several types of cells. The effects of blue LEDs have not been tested on bone marrow-derived mesenchymal stem cells (BMSCs), which are important for cell-based therapy in various medical fields. Therefore, the aim of this study was to determine the effects of blue LED on the proliferation, apoptosis and osteogenic differentiation of BMSCs. METHODS: BMSCs were irradiated with a blue LED light at 470 nm for 1 min, 5 min, 10 min, 30 min and 60 min or not irradiated. Cell proliferation was measured by performing cell counting and EdU staining assays. Cell apoptosis was detected by TUNEL staining. Osteogenic differentiation was evaluated by ALP and ARS staining. DCFH-DA staining and γ-H2A.X immunostaining were used to measure intracellular levels of ROS production and DNA damage. RESULTS: Both cell counting and EdU staining assays showed that cell proliferation of BMSCs was significantly reduced upon blue LED irradiation. Furthermore, treatment of BMSCs with LED irradiation was followed by a remarkable increase in apoptosis, indicating that blue LED light induced toxic effects on BMSCs. Likewise, BMSC osteogenic differentiation was inhibited after exposure to blue LED irradiation. Further, blue LED irradiation was followed by the accumulation of ROS production and DNA damage. CONCLUSIONS: Taken together, our study demonstrated that blue LED light inhibited cell proliferation, inhibited osteogenic differentiation, and induced apoptosis in BMSCs, which are associated with increased ROS production and DNA damage. These findings may provide important insights for the application of LEDs in future BMSC-based therapies.
BACKGROUND/AIMS: Blue light emitting diodes (LEDs) have been proven to affect the growth of several types of cells. The effects of blue LEDs have not been tested on bone marrow-derived mesenchymal stem cells (BMSCs), which are important for cell-based therapy in various medical fields. Therefore, the aim of this study was to determine the effects of blue LED on the proliferation, apoptosis and osteogenic differentiation of BMSCs. METHODS: BMSCs were irradiated with a blue LED light at 470 nm for 1 min, 5 min, 10 min, 30 min and 60 min or not irradiated. Cell proliferation was measured by performing cell counting and EdU staining assays. Cell apoptosis was detected by TUNEL staining. Osteogenic differentiation was evaluated by ALP and ARS staining. DCFH-DA staining and γ-H2A.X immunostaining were used to measure intracellular levels of ROS production and DNA damage. RESULTS: Both cell counting and EdU staining assays showed that cell proliferation of BMSCs was significantly reduced upon blue LED irradiation. Furthermore, treatment of BMSCs with LED irradiation was followed by a remarkable increase in apoptosis, indicating that blue LED light induced toxic effects on BMSCs. Likewise, BMSC osteogenic differentiation was inhibited after exposure to blue LED irradiation. Further, blue LED irradiation was followed by the accumulation of ROS production and DNA damage. CONCLUSIONS: Taken together, our study demonstrated that blue LED light inhibited cell proliferation, inhibited osteogenic differentiation, and induced apoptosis in BMSCs, which are associated with increased ROS production and DNA damage. These findings may provide important insights for the application of LEDs in future BMSC-based therapies.
Authors: Sherif A Mohamad; Michael R Milward; Mohammed A Hadis; Sarah A Kuehne; Paul R Cooper Journal: Photochem Photobiol Sci Date: 2021-05-04 Impact factor: 3.982
Authors: Hannah Serrage; Vladimir Heiskanen; William M Palin; Paul R Cooper; Michael R Milward; Mohammed Hadis; Michael R Hamblin Journal: Photochem Photobiol Sci Date: 2019-06-11 Impact factor: 3.982
Authors: Barbara Sampaio Dias Martins Mansano; Vitor Pocani da Rocha; Ednei Luiz Antonio; Daniele Fernanda Peron; Rafael do Nascimento de Lima; Paulo Jose Ferreira Tucci; Andrey Jorge Serra Journal: Oxid Med Cell Longev Date: 2021-02-03 Impact factor: 6.543